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Production of Green Fluorescent Protein in Transgenic Rice Seeds

文献类型： 外文期刊

作者： Li, Ding 1 ; Gao, Jing 1 ; Shen, Chunxiu 1 ; Fang, Zhen 1 ; Xia, Yumei 1 ; Yuan, Longping 2 ; Cao, Mengliang 1 ;

作者机构： 1.Cent S Univ, Sch Longping, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China

2.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China

3.Hunan Longping Gene Co Ltd, Changsha 410125, Hunan, Peoples R China

关键词： Callus;Transgenic Rice;Green Fluorescent Seeds

期刊名称：JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY （ 影响因子：1.134； 五年影响因子：0.999 ）

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收录情况： SCI

摘要： Immature embryos from immature seeds of rice (Oryza sativa L.) were transformed by biolistic bombardment with the plasmid carrying the coding region of the hygromycin phosphotransferase gene under the control of the 5' region of the cauliflower mosaic virus 35S promoter and the synthetic green fluorescence protein gene (sgfp) under the control of the maize ubiquitine promoter. Southern blot analysis confirmed the stable integration of hpt and sgfp genes in transformants. Subsequently leaves from regenerated plants were resistant to hygromycin, and microscopic observation of the green fluorescence and immunoblotting analysis revealed that green fluorescence protein was not only detected in the leaf and pollen of primary transformants but also in mature seeds. The results bear out the importance of the suitability of GFP as an in vivo marker to follow the processes of selection of somatic hybrid embryos and plants.

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