The CYP392D8 gene is not directly associated with abamectin resistance, a case study in two highly resistant Tetranychus urticae strains
文献类型： 外文期刊
作者： Xu, Dandan 1 ; Liao, Haojie 1 ; Li, Lingyun 1 ; Wu, Mingmei 1 ; Xie, Wen 1 ; Wu, Qingjun 1 ; Zhang, Youjun 1 ; Zhou, Xiaomao 2 ; Wang, Shaoli 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
2.Grad Sch Hunan Univ, Longping Branch, Changsha 410125, Peoples R China
3.Hunan Acad Agr Sci, Hunan Agr Biotechnol Res Inst, Changsha 410125, Peoples R China
关键词： Pesticide resistance; P450 expression; transgenic Drosophila; heterologous expression; in -vitro metabolism
期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：6.608； 五年影响因子：4.803 ）
ISSN： 0171-8177
年卷期：
页码：
收录情况： SCI
摘要： Overuse of the pesticide abamectin has resulted in many abamectin-resistant populations of the spider mite Tetranychus urticae, which is an important agricultural pest. Abamectin resistance has been predicted to be related to an overexpressed cytochrome P450 gene, CYP392D8. However, whether CYP392D8 truly contributes to abamectin resistance in T. urticae is unknown. To determine whether there is a functional link between CYP392D8-mediated metabolism and abamectin resistance, we cloned the CDS sequence of CYP392D8 and two variants among susceptible and resistant T. urtice populations were observed. We found that CYP392D8 was overexpressed in two abamectin-resistant populations and was induced following exposure to an LC30 concentration of abamectin. However, the heterologous expression and transgenic Drosophila melanogaster indicated that the two CYP392D8 variants could not metabolize abamectin and that enhanced expression of CYP392D8 was not sufficient to increase tolerance to abamectin. Taken together, our findings demonstrate that CYP392D8 does not contribute to abamectin resistance in two Chinese T. urticae strains directly.
- 相关文献
作者其他论文 更多>>
-
RNAi-mediated knockdown of fruitless in Plutella xylostella (Lepidoptera: Plutellidae) disrupts female sex pheromone biosynthesis and male courtship behavior
作者：Huang, Zizhou;Ma, Haihao;Liu, Jia;Zhou, Xiaomao;Liu, Zheming;Ma, Haihao;Zhou, Yong;Zhao, Piao;Zhu, Hang;Liu, Jia;Man, Yilong;Liu, Zheming;Ma, Haihao;Zhou, Yong;Zhao, Piao;Zhu, Hang;Liu, Jia;Man, Yilong;Zhou, Xiaomao;Liu, Zheming;Ma, Haihao;Zhou, Yong;Zhao, Piao;Zhu, Hang;Liu, Jia;Man, Yilong;Zhou, Xiaomao;Zhou, Xiaomao
关键词：fruitless; alternative splicing; courtship behavior; sex pheromone
-
Genetic and pharmacological inhibition of Kir1 impacts mating behavior of the diamondback moth (Plutella xylostella)
作者：Huo, Xiaoyi;Zhou, Xiaomao;Huo, Xiaoyi;Zhou, Xiaomao;Liu, Jia;Ma, Haihao;Zhou, Xiaomao;Liu, Zheming;Zhao, Piao;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhang, Chengjia;Ma, Haihao;Zhou, Xiaomao;Liu, Zheming;Zhao, Piao;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhang, Chengjia;Ma, Haihao;Zhou, Xiaomao;Liu, Zheming;Zhao, Piao;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhang, Chengjia;Ma, Haihao
关键词：Plutella xylostella; Kir1 channel; mating; pest management
-
The Elongation Factor 1 Alpha Promoter Drives the Functional Expression of Kir2A in Plutella xylostella Cells
作者：Wang, Yinna;Ma, Haihao;Zhou, Xiaomao;Wang, Yinna;Zhou, Xiaomao;Ma, Haihao;Liu, Zheming;Zhao, Piao;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Ma, Haihao;Liu, Zheming;Zhao, Piao;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao;Ma, Haihao;Liu, Zheming;Zhao, Piao;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao
关键词：
Plutella xylostella ; EF1 alpha promoter; Kir2 channel; cell line -
Transcriptome Analysis to Identify Resistance Genes Associated With Tomato Varieties in Response to MED Bemisia tabaci (Hemiptera: Aleyrodidae)
作者：Kong, Hailong;Chen, Yuxuan;Hu, Rongli;Muhammad, Taimur;Jiang, Suwan;Chen, Zhiwei;Zhang, Xi;Fan, Zicheng;Luo, Chen;Shi, Xiaobin;Wu, Qingjun;Zhang, Youjun
关键词：Bemisia tabaci; plant defence response; tomato varieties; transcription factors; transcriptome analysis
-
GPCR-MAPK signaling pathways underpin fitness trade- offs in whitefly
作者：Fu, Buli;Liang, Jinjin;Hu, Jinyu;Du, Tianhua;He, Chao;Wei, Xuegao;Gong, Peipan;Yang, Jing;Liu, Shaonan;Huang, Mingjiao;Yang, Xin;Zhang, Youjun;Fu, Buli;Hu, Jinyu;Liu, Kui;Du, Tianhua;Wei, Xuegao;Gui, Lianyou;Zhang, Youjun;Tan, Qimei;He, Chao;Liu, Shaonan;Huang, Mingjiao;Zhang, Youjun;Wei, Xuegao;Nauen, Ralf;Bass, Chris
关键词：adaptive evolution; fitness trade- offs; GPCR; MAPK; P450
-
Electrophysiological and pharmacological properties of the slowpoke channel in the diamondback moth, Plutella xylostella
作者：Huo, Xiaoyi;Liu, Zheming;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao;Ma, Haihao;Huo, Xiaoyi;Wang, Yinna;Zhou, Xiaomao;Ma, Haihao;Liu, Zheming;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao;Ma, Haihao;Liu, Zheming;Liu, Jia;Zhu, Hang;Zhou, Yong;Zhou, Xiaomao;Ma, Haihao
关键词：Plutella xylostella; Slowpoke channel; Patch clamp; Electrophysiology; Inhibitor
-
Frequencies of insecticide resistance mutations detected by the amplicon sequencing in Plutella xylostella (Lepidoptera: Plutellidae) and Spodoptera exigua (Lepidoptera: Noctuidae) from China
作者：Liu, Zhangyang;Ma, Haihao;Li, Kaiqin;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao;Liu, Zheming;Liu, Zhangyang;Ma, Haihao;Li, Kaiqin;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhou, Xiaomao;Liu, Zheming;Liu, Zhangyang;Li, Kaiqin;Zhou, Xiaomao
关键词：Plutella xylostella; Spodoptera exigua; insecticide resistance; mutation detection; amplicon sequencing
