您好，欢迎访问湖南省农业科学院 机构知识库！

A Molecular Recruitment Colocalization Platform for Visualizing Multi-Protein Interactions and Engineering Biomolecular Condensates in Living Cells

文献类型： 外文期刊

作者： Peng, Lei 1 ; Hou, Yu 1 ; Zhao, Yan 4 ; Tang, Jing-Ya 1 ; Song, Liu 1 ; Peng, Bo 1 ; Li, Min 1 ; Wang, Dian-Bing 1 ; Zhang, Xian-En 1 ;

作者机构： 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China

2.Guizhou Normal Univ, Coll Life Sci, Guiyang 550025, Peoples R China

3.Shenzhen Univ Adv Technol, Fac Synthet Biol, Shenzhen 518107, Peoples R China

4.Hunan Acad Agr Sci, Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China

关键词： colocalization; condensates; live cell imaging; multi-protein interactions; phase separation; protein binding ability

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

ISSN：

年卷期： 2025 年

页码：

收录情况： SCI

摘要： Visualizing dynamic protein-protein interactions (PPIs) and phase-separated biomolecular condensates in live cells is crucial for understanding cellular processes. Here, a method is introduced for analyzing both PPIs and condensates, termed molecular recruitment colocalization (MRC), which localizes interacting partners and generates fluorescent signals at designated genomic loci. MRC enables the simultaneous visualization of multi-protein interactions and the preliminary estimation of binding affinities during PPIs. Through MRC, the study can quickly discover and validate new proteins or structural domains capable of forming condensates and can also integrate PPIs to study condensates. Furthermore, the versatility of MRC is demonstrated in phase-separated condensates research: programming phase separation sites to study the interactions and behaviors between different condensates, recruiting target proteins into programmable condensates, obtaining the properties of diverse engineered condensates, and probing the physical properties of condensates within live cells. Therefore, MRC can become a versatile platform for studying both PPIs and biomolecular condensates.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台