Insights into the physiological, molecular, and genetic regulators of albinism in Camellia sinensis leaves
文献类型: 外文期刊
作者: Zhao, Yang 1 ; Yang, Peidi 1 ; Cheng, Yang 1 ; Liu, Yong 1 ; Yang, Yang 1 ; Liu, Zhen 1 ;
作者机构: 1.Hunan Acad Agr Sci, Tea Res Inst, Changsha, Hunan, Peoples R China
关键词: albinism; leaves; pigmentation; flavonoids; carotenoids; chlorophyll
期刊名称:FRONTIERS IN GENETICS ( 影响因子:3.7; 五年影响因子:4.3 )
ISSN:
年卷期: 2023 年 14 卷
页码:
收录情况: SCI
摘要: Introduction: Yanling Yinbiancha, a cultivar of Camellia sinensis (L.) O. Kuntze, is an evergreen woody perennial with characteristic albino leaves. A mutant variant with green leaves on branches has been recently identified. The molecular mechanisms underlying this color variation remain unknown.Methods: We aimed to utilize omics tools to decipher the molecular basis for this color variation, with the ultimate goal of enhancing existing germplasm and utilizing it in future breeding programs.Results and discussion: Albinotic leaves exhibited significant chloroplast degeneration and reduced carotenoid accumulation. Transcriptomic and metabolomic analysis of the two variants revealed 1,412 differentially expressed genes and 127 differentially accumulated metabolites (DAMs). Enrichment analysis for DEGs suggested significant enrichment of pathways involved in the biosynthesis of anthocyanins, porphyrin, chlorophyll, and carotenoids. To further narrow down the causal variation for albinotic leaves, we performed a conjoint analysis of metabolome and transcriptome and identified putative candidate genes responsible for albinism in C. sinensis leaves. 12, 7, and 28 DEGs were significantly associated with photosynthesis, porphyrin/chlorophyll metabolism, and flavonoid metabolism, respectively. Chlorophyllase 2, Chlorophyll a-Binding Protein 4A, Chlorophyll a-Binding Protein 24, Stay Green Regulator, Photosystem II Cytochrome b559 subunit beta along with transcription factors AP2, bZIP, MYB, and WRKY were identified as a potential regulator of albinism in Yanling Yinbiancha. Moreover, we identified Anthocyanidin reductase and Arabidopsis Response Regulator 1 as DEGs influencing flavonoid accumulation in albino leaves. Identification of genes related to albinism in C. sinensis may facilitate genetic modification or development of molecular markers, potentially enhancing cultivation efficiency and expanding the germplasm for utilization in breeding programs.
- 相关文献
作者其他论文 更多>>
-
Tomato chlorosis virus CPm protein is a pathogenicity determinant and suppresses host local RNA silencing induced by single-stranded RNA
作者:Yang, Xiao;Luo, Xiangwen;Zhang, Zhanhong;Shi, Xiaobin;Zhang, Songbai;Liu, Yong;Zhang, Deyong;Luo, Xiangwen;Zhang, Yu;Zhang, Zhanhong;OuYang, Xian;Shi, Xiaobin;Zhang, Songbai;Liu, Yong;Zhang, Deyong;Lv, Xiaoyuan;Li, Fan
关键词:Tomato chlorosis virus; CPm protein; pathogenicity determinant; RNA silencing suppressor; posttranscriptional gene silencing
-
CRISPR/Cas9-mediated gene editing of vacuolar ATPase subunit d mediates phytohormone biosynthesis and virus resistance in rice
作者:Lu, Qinghua;Luo, Xiangwen;Yang, Xiao;Zhang, Deyong;Li, Yixin;Li, Li;Zhang, Songbai;Liu, Yong;Luo, Xiangwen;Zhang, Yu;Zhang, Deyong;Zheng, Limin;Li, Yixin;Li, Li;Zhang, Songbai;Liu, Yong;Zhou, Tong;Lan, Ying
关键词:CRISP; Cas9; vacuolar ATPase subunit d; phytohormone biosynthesis; virus resistance; rice
-
Vector-mediated viral exchange between crops and weedy plants
作者:Shi, Xiao-bin;Zhang, Zhuo;Huang, Li-ping;Zhang, De-yong;Zhang, Zhan-hong;Zhang, Song-bai;Liu, Yong;Li, Fan;Huang, Li-ping;Preisser, Evan L.;Zhou, Xu-guo;Zhang, An-sheng;Liu, Hui;Wu, Yuan-hua;Cheng, Xiao-fei;He, Zi-fu
关键词:Weeds; Alternative host; Bemisia tabaci; Tomato chlorosis virus; Transmission
-
Tomato Chlorosis Virus (ToCV) Infection Induced the Resistance of Bemisia tabaci to Two Insecticides: Pyrethroids and Flupyradifurone
作者:Zhang, Jisong;Zhang, Youjun;Huang, Liping;Zhang, Zhuo;Zhang, Zhanhong;Zhang, Deyong;Shi, Xiaobin;Liu, Yong;Zhang, Youjun
关键词:Bemisia tabaci MED; ToCV; insecticide resistance
-
Effects of insulin-like peptide 7 in Bemisia tabaci MED on tomato chlorosis virus transmission
作者:Xu, HuiNan;Zhang, DeYong;Shi, XiaoBin;Liu, Yong;Xu, HuiNan;Zhang, Zhuo;Peng, Jing;Gao, Yang;Li, KaiLong;Chen, Jianbin;Du, Jiao;Yan, Shuo;Zhang, DeYong;Shi, XiaoBin;Liu, Yong;Zhang, ZhanHong;Zhou, XuGuo
关键词:Bemisia tabaci MED; tomato chlorosis virus; trehalose metabolism; insulin-like peptide 7; RNA interference
-
Investigating the antidepressant effect of Ziyan green tea on chronic unpredictable mild stress mice through fecal metabolomics
作者:Jia, Wenbao;Tang, Qian;Zou, Yao;Xu, Wei;Jia, Wenbao;Wu, Wenliang;Yang, Yang
关键词:Ziyan green tea; CUMS; depression-like behaviors; neurochemical factors; pro-inflammatory factors; gut microbiota; fecal metabolites
-
Co-infection of TYLCV and ToCV increases cathepsin B and promotes ToCV transmission by Bemisia tabaci MED
作者:Lu, Ding-Yi-Hui;Shi, Xiao-Bin;Liu, Yong;Zhang, De-Yong;Lu, Ding-Yi-Hui;Liao, Jin-Yu;Chen, Jian-Bin;Wei, Yan;Zhang, Zhuo;Zheng, Li-Min;Tan, Xin-Qiu;Shi, Xiao-Bin;Liu, Yong;Zhang, De-Yong;Fajar, Anugerah;Zhou, Xu-Guo;Fajar, Anugerah;Zhang, Zhan-Hong
关键词:Bemisia tabaci; tomato chlorosis virus; tomato yellow leaf curl virus; single infection; co-infection