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Identification and Expression Analysis of Hexokinases Family in Saccharum spontaneum L. under Drought and Cold Stresses

文献类型： 外文期刊

作者： Liu, Ying 1 ; Jiang, Yaolan 1 ; Liu, Xiaolan 1 ; Cheng, Hefen 1 ; Han, Yuekun 1 ; Zhang, Dawei 1 ; Wu, Jinfeng 1 ; Liu, Lili 1 ; Yan, Mingli 1 ; Que, Youxiong 3 ; Zhou, Dinggang 1 ;

作者机构： 1.Hunan Univ Sci & Technol, Sch Life & Hlth Sci, Hunan Key Lab Econ Crops Genet Improvement & Integ, Xiangtan 411201, Peoples R China

2.Hunan Acad Agr Sci, Crop Res Inst, Changsha 410000, Peoples R China

3.Fujian Agr & Forestry Univ, Minist Agr, Minist Sci & Technol, Natl Engn Res Ctr Sugarcane,Key Lab Sugarcane Biol, Fuzhou 350002, Peoples R China

4.Coll Hunan Prov, Key Lab Ecol Remediat & Safe Utilizat Heavy Met Po, Xiangtan 411201, Peoples R China

关键词： Saccharum spontaneum; hexokinase; genome-wide analysis; transcriptome; expression patterns

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 12 卷 6 期

页码：

收录情况： SCI

摘要： In plants, the multi-gene family of dual-function hexokinases (HXKs) plays an important role in sugar metabolism and sensing, that affects growth and stress adaptation. Sugarcane is an important sucrose crop and biofuel crop. However, little is known about the HXK gene family in sugarcane. A comprehensive survey of sugarcane HXKs, including physicochemical properties, chromosomal distribution, conserved motifs, and gene structure was conducted, identifying 20 members of the SsHXK gene family that were located on seven of the 32 Saccharum spontaneum L. chromosomes. Phylogenetic analysis showed that the SsHXK family could be divided into three subfamilies (group I, II and III). Motifs and gene structure were related to the classification of SsHXKs. Most SsHXKs contained 8-11 introns which was consistent with other monocots. Duplication event analysis indicated that HXKs in S. spontaneum L. primarily originated from segmental duplication. We also identified putative cis-elements in the SsHXK promoter regions which were involved in phytohormone, light and abiotic stress responses (drought, cold et al.). During normal growth and development, 17 SsHXKs were constitutively expressed in all ten tissues. Among them, SsHXK2, SsHXK12 and SsHXK14 had similar expression patterns and were more highly expressed than other genes at all times. The RNA-seq analysis showed that 14/20 SsHXKs had the highest expression level after cold stress for 6 h, especially SsHXK15, SsHXK16 and SsHXK18. As for drought treatment, 7/20 SsHXKs had the highest expression level after drought stress for 10 days, 3/20 (SsHKX1, SsHKX10 and SsHKX11) had the highest expression level after 10 days of recovery. Overall, our results revealed the potential biological function of SsHXKs, which may provide information for in-depth functional verification.

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