Pharmacological Properties of the Type 1 Tyramine Receptor in the Diamondback Moth, Plutella xylostella
文献类型： 外文期刊
作者： Ma, Haihao 1 ; Huang, Qingting 1 ; Lai, Xiaoyi 1 ; Liu, Jia 1 ; Zhu, Hang 1 ; Zhou, Yong 1 ; Deng, Xile 1 ; Zhou, Xiaomao 1 ;
作者机构： 1.Hunan Acad Agr Sci, Inst Agr Biotechnol, Changsha 410125, Hunan, Peoples R China
关键词： tyramine receptor; P; xylostella; agonist; antagonist; gene expression
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN： 1422-0067
年卷期： 2019 年 20 卷 12 期
页码：
收录情况： SCI
摘要： Tyramine receptors (TARs) can be activated by tyramine (TA) or octopamine (OA) and have been shown to be related to physiological regulation (e.g., gustatory responsiveness, social organization, and learning behavior) in a range of insect species. A tyramine receptor gene in Plutella xylostella, Pxtar1, was cloned and stably expressed in the HEK-293 cell line. Pharmacological properties and expression profile of Pxtar1 were also analyzed. Tyramine could activate the PxTAR1 receptor, increasing the intracellular Ca2+ concentration ((Ca2+)i) at an EC50 of 13.1 nM and reducing forskolin (10 mu M)-stimulated intracellular cAMP concentration ((cAMP)i) at an IC50 of 446 nM. DPMF (a metabolite of amitraz) and L(-)-carvone (an essential oil) were found to act as PxTAR1 receptor agonists. Conversely, yohimbine and mianserin had significant antagonistic effects on PxTAR1. In both larvae and adults, Pxtar1 had the highest expression in the head capsule and expression of Pxtar1 was higher in male than in female reproductive organs. This study reveals the temporal and spatial differences and pharmacological properties of Pxtar1 in P. xylostella and provides a strategy for screening insecticidal compounds that target PxTAR1.
- 相关文献
作者其他论文 更多>>
-
A Supramolecular Material for Controlling Kiwifruit Bacterial Canker
作者：Deng, Xile;Bai, Lianyang;Zhang, Jichuan;Liu, Tianqi;Zhang, Jiaheng;Bian, Qiang;Zhang, Yizhuo;Zhang, Li;Zhou, Mingqing;Xie, Le
关键词：bactericidal activity; kiwifruit bacterial canker (KBC); molecular simulations; nano pesticide; supramolecular nanocarrier
-
A Novel Salicylic Rice Safener Fulfilled through Supramolecular Capsulation
作者：Deng, Xile;Deng, Yanan;Peng, Yajun;Bai, Lianyang;Zhang, Yong;Zhang, Jichuan;Liu, Tianqi;Zhang, Jiaheng;Dong, Zhichao
关键词：environmental security; ion salt; nanoencapsulation; rice; safener
-
RNAi-mediated knockdown of fruitless in Plutella xylostella (Lepidoptera: Plutellidae) disrupts female sex pheromone biosynthesis and male courtship behavior
作者：Huang, Zizhou;Ma, Haihao;Liu, Jia;Zhou, Xiaomao;Liu, Zheming;Ma, Haihao;Zhou, Yong;Zhao, Piao;Zhu, Hang;Liu, Jia;Man, Yilong;Liu, Zheming;Ma, Haihao;Zhou, Yong;Zhao, Piao;Zhu, Hang;Liu, Jia;Man, Yilong;Zhou, Xiaomao;Liu, Zheming;Ma, Haihao;Zhou, Yong;Zhao, Piao;Zhu, Hang;Liu, Jia;Man, Yilong;Zhou, Xiaomao;Zhou, Xiaomao
关键词：fruitless; alternative splicing; courtship behavior; sex pheromone
-
Preparation of a smart nano-herbicide MP@HLDP with strong permeability performance improves herbicidal activity while reducing the adverse impacts
作者：Deng, Xile;Dong, Zhichao;Bai, Lianyang;Deng, Xile;Dong, Zhichao;Bai, Lianyang;Wang, Zeng;Yin, Jiaming;Shen, Jie;Yan, Shuo;Dong, Zhichao
关键词：Enhanced bioactivity; HLDP; Nano-delivery system; Nano-pesticide; Strong adhesion performance
-
Multi-omics analyses reveal the mechanisms of developmental toxicity of a covalent organic framework to the roots of rice (Oryza sativa) seedlings
作者：Deng, Xile;Deng, Xile;Xu, Yujun;Deng, Yanan;Yu, Simin;Deng, Xile;Yu, Simin;Qian, Le
关键词：
-
Differential ecotoxicity of nanoencapsulated metamifop and its active ingredient in zebrafish (Danio rerio): Insights from transcriptome and metabolome analysis
作者：Xu, Yujun;Yu, Simin;Deng, Xile;Yin, Jiaming;Wang, Zeng;Shen, Jie;Yan, Shuo;Liu, Shaowen
关键词：Nanopesticide; Zebrafish; MP@HLDP; Apoptosis; Cytochrome P450; Detoxification
-
Genetic and pharmacological inhibition of Kir1 impacts mating behavior of the diamondback moth (Plutella xylostella)
作者：Huo, Xiaoyi;Zhou, Xiaomao;Huo, Xiaoyi;Zhou, Xiaomao;Liu, Jia;Ma, Haihao;Zhou, Xiaomao;Liu, Zheming;Zhao, Piao;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhang, Chengjia;Ma, Haihao;Zhou, Xiaomao;Liu, Zheming;Zhao, Piao;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhang, Chengjia;Ma, Haihao;Zhou, Xiaomao;Liu, Zheming;Zhao, Piao;Liu, Jia;Zhu, Hang;Zhou, Yong;Man, Yilong;Zhang, Chengjia;Ma, Haihao
关键词：Plutella xylostella; Kir1 channel; mating; pest management
