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Lotus seed skin proanthocyanidin extract exhibits potent antioxidant property via activation of the Nrf2-ARE pathway

文献类型： 外文期刊

作者： Li, Tao 1 ; Li, Qili 2 ; Wu, Weiguo 1 ; Li, Yong 1 ; Hou, De-xing 1 ; Xu, Hua 4 ; Zheng, Baodong 5 ; Zeng, Shaoxiao 5 ; Shan 1 ;

作者机构： 1.Hunan Agr Univ, Coll Food Sci & Technol, Key Lab Food Sci & Biotechnol Hunan Prov, Core Res Program 1515, Changsha 410128, Hunan, Peoples R China

2.Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Changsha 410125, Hunan, Peoples R China

3.Kagoshima Univ, United Grad Sch Agr Sci, Fac Agr, Kagoshima 8900065, Japan

4.Univ Arizona, Dept Pediat, Steele Childrens Res Ctr, Tucson, AZ 85724 USA

5.Fujian Agr & Forestry Univ, Dept Food Safety, Coll Food Sci, Fuzhou 350002, Fujian, Peoples R China

关键词： lotus seed skin extract; proanthocyanidin; Nrf2-ARE pathway; antioxidant activity

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 1672-9145

年卷期： 2019 年 51 卷 1 期

页码：

收录情况： SCI

摘要： Lotus seed is well known as traditional food and medicine, but its skin is usually discarded. Recent studies have shown that lotus seed skin contains a high concentration of proanthocyanidins that have multi-functions, such as antioxidation, anti-inflammation, and anti-cancer effects. In the present study, we aimed to isolate and purify the proanthocyanidins from lotus seed skin by acetone extraction and rotary evaporation, identify their chemical structures by HPLC-MS-MS and NMR, and further investigate the antioxidant properties of the extract purified by macroporous resin (PMR) from lotus seed skin both in vitro and in vivo. The results showed that PMR mainly contained oligomeric proanthocyanidins, especially dimeric procyanidin B1 (PB1), procyanidin B2 and procyanidin B4. Although it had limited ability to directly scavenge radicals in vitro, PMR could significantly enhance the expressions of antioxidant proteins via activation of nuclear factor-E2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway in HepG2 cells. Molecular data revealed that PB1, a major component in PMR, stabilized Nrf2 by inhibiting the ubiquitination of Nrf2, which led to subsequent activation of the Nrf2-ARE pathway, including the enhancements of Nrf2 nuclear translocation, Nrf2-ARE binding and ARE transcriptional activity. Moreover, the in vivo results in high fat diet-induced mice further verified the powerful antioxidant property of PMR. These results revealed that lotus seed skin is a promising resource for functional food development.

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