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OsPKS2 is required for rice male fertility by participating in pollen wall formation

文献类型： 外文期刊

作者： Zou, Ting 2 ; Liu, Mingxing 1 ; Xiao, Qiao 1 ; Wang, Tao 1 ; Chen, Dan 1 ; Luo, Tao 1 ; Yuan, Guoqiang 1 ; Li, Qiao 1 ; Zhu, 1 ;

作者机构： 1.Sichuan Agr Univ, State Key Lab Hybrid Rice, Chengdu 611130, Sichuan, Peoples R China

2.Sichuan Agr Univ, Rice Res Inst, 211 Huimin Rd, Wenjiang 611130, Sichuan, Peoples R China

3.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China

4.Chongqing Univ Arts & Sci, Inst Special Plants, Collaborat Innovat Ctr Special Plant Ind Chongqin, Chongqing Key Lab Econ Plant Biotechnol, Yongchuan 402160, Peoples R China

关键词： OsPKS2; PKSB/LAP5; Rice; Pollen; Exine; Sporopollenin

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2018 年 37 卷 5 期

页码：

收录情况： SCI

摘要： OsPKS2, the rice orthologous gene of Arabidopsis PKSB/LAP5, encodes a polyketide synthase that is involved in pollen wall formation in rice. In flowering plants, the pollen wall protects male gametes from various environmental stresses and pathogen attacks, as well as promotes pollen germination. The biosynthesis of sporopollenin in tapetal cell is critical for pollen wall formation. Recently, progress has been made in understanding sporopollenin metabolism during pollen wall development in Arabidopsis. However, little is known about the molecular mechanism that underlies the sporopollenin synthesis in pollen wall formation in rice (Oryza sativa). In this study, we identified that a point mutation in OsPKS2, a plant-specific type III polyketide synthase gene, caused male sterility in rice by affecting the normal progress of pollen wall formation. Two other allelic mutants of OsPKS2 were generated using the CRISPR/Cas9 system and are also completely male sterile. This result thus further confirmed that OsPKS2 controls rice male fertility. We also showed that OsPKS2 is an orthologous gene of Arabidopsis PKSB/LAP5 and has a tapetum-specific expression pattern. In addition, its product localizes in the endoplasmic reticulum. Results suggested that OsPKS2 is critical for pollen wall formation, and plays a conserved but differentiated role in sporopollenin biosynthesis from Arabidopsis.

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