Deep sequencing analysis of a strain of pecan mosaic-associated virus infecting Atractylodes macrocephala Koidz
文献类型： 外文期刊
作者： Niu, Yanbing 1 ; Pang, Xiaojing 1 ; Wang, Defu 1 ; Guo, Shang 2 ; Liu, Yong 3 ;
作者机构： 1.Shanxi Agr Univ, Coll Life Sci, 01 Mingxian Rd, Taigu 030801, Peoples R China
2.Shanxi Acad Agr Sci, Inst Edible Fungi, Taiyuan 030006, Peoples R China
3.Hunan Acad Agr Sci, Key Lab Pest Management Hort Crop Hunan Prov, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China
关键词： Atractylodes macrocephala Koidz; Pecan mosaic-associated virus; Virus-derived small interfering RNAs; Deep sequencing
期刊名称：JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.729； 五年影响因子：1.681 ）
ISSN： 1125-4653
年卷期： 2018 年 100 卷 2 期
页码：
收录情况： SCI
摘要： Leaves of Atractylodes macrocephala Koidz with virus-like symptoms were collected in Shanxi Province, China. The strain Am of the Potyvirus species, pecan mosaic-associated virus (PMaV-Am), was discovered through the assembly of virus derived small interfering RNAs (vsiRNAs) that were isolated from symptomatic samples. The complete genome of PMaV-Am was determined to be 9310 nucleotides (nt) in length, including a large open reading frame encoding a polyprotein. Phylogenetic analysis showed that PMaV-Am is clustered together and most closely related to strain PMaV-LA from pecan. Sequence alignments of PMaV-Am complete genomic RNAs with PMaV-LA showed 98.78% nucleotide sequence homology and 96.53% amino acid identity, respectively. Further analyses of the vsiRNA profile showed that the most abundant PMaV from A. macrocephala (PMaV-Am) vsiRNAs were 21 nt long and biased for A at their 5' terminal residue. Polarity distribution revealed that vsiRNAs were nearly equal amount of sense and antisense strands and hotspot was distributed in the HC-Pro coding region of the PMaV-Am genome. These results might provide an insight into the host RNA silencing defense induced by PMaV-Am, and provide guidelines on designing antiviral strategies using RNAi against PMaV-Am.
- 相关文献
作者其他论文 更多>>
-
Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag Nanoparticles
作者：Ning, Weimin;Li, Mingxuan;Jiang, Lei;Yang, Mei;Liu, Maoyan;Ning, Weimin;Liu, Yong;Liu, Yong
关键词：endophytic bacteria; Cu-Ag nanoparticles; bacterial community; BLB; rice
-
Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function
作者：Gong, Pan;Gao, Mengxin;Chen, Yalin;Zhang, Mingzhen;Hu, Xiaohua;Zhao, Siwen;Pan, Mengjiao;Cao, Buwei;Zhou, Xueping;Li, Fangfang;Gao, Mengxin;Zhou, Xueping;Huang, Yucong;Shen, Qingtang;Zhang, Hui;Liu, Yong;Liu, Yong;Lozano-Duran, Rosa;Wang, Aiming
关键词：positive-sense single-stranded RNA viruses; tobamoviruses; cucumber green mottle mosaic virus; small ORFs; peroxisomes; virus pathogenicity
-
Host metabolites explain microbiome variation between different rice genotypes
作者：Su, Pin;Peng, Weiye;Sun, Shu'e;Du, Xiaohua;Bai, Lianyang;Liu, Yong;Zhang, Deyong;Su, Pin;Peng, Weiye;Sun, Shu'e;Du, Xiaohua;Bai, Lianyang;Liu, Yong;Zhang, Deyong;Kang, Houxiang;Peng, Qianze;Zhang, Deyong;Peng, Qianze;Zhang, Deyong;Zhang, Chi;Lei, Ziling;Liu, Yong;Zhang, Deyong;Tang, Qianjun;Cernava, Tomislav
关键词：Plant genotype; Plant microbiome; Genetic control; Plant metabolites; Phenylpropanoids
-
Analysis of the Antagonistic Effect of Dibutyl Phthalate on Magnaporthe oryzae through Bioassay and Omics Data
作者：Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue
关键词：dibutyl phthalate; antifungal activity; Magnaportheoryzae; transcriptome; metabolome
-
Metabolomic Profiling Reveals the Effects of Cu-Ag Nanoparticles on Tomato Bacterial Wilt
作者：Ning, Weimin;Jiang, Lei;Yang, Mei;Lei, Tianhao;Liu, Chan;Zhao, Fei;Shu, Pan;Ning, Weimin;Liu, Yong;Ning, Weimin;Liu, Yong
关键词：Cu-Ag nanoparticles; tomato; metabolomics; fatty acyls; organooxygen compounds
-
Transcriptome Analysis Reveals the Role of OsCBM1 in Rice Defense Against Xanthomonas oryzae pv.oryzae
作者：Bie, Shuaijun;Liu, Yong;Zhang, Deyong;Xiao, Youlun;Liu, Yong;Peng, Jing;Gao, Yang;Li, Xiaojuan;Tan, Xinqiu;Huang, Renyan;Zhang, Deyong;Zhang, Li;He, Xiaomin;Xie, Hongjun;Huang, Renyan;Zhang, Deyong
关键词：malectin-like domain-containing protein;
OsCBM1 ;Xanthomonas oryzae pv. oryzae ; reactive oxygen species; phytohormones; rice -
A viral movement protein mediates host volatile biosynthesis to co-attract vectors and non-vectors and enhances viral infection
作者：Li, Shijun;Luo, Xiangwen;Zhang, Zhanhong;Ouyang, Xian;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Luo, Xiangwen;Zhang, Yu;Zhang, Zhanhong;Yang, Xiao;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Luo, Xiangwen;Zhang, Yu;Zhang, Zhanhong;Yang, Xiao;Ouyang, Xian;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Zhou, Xuguo;Wang, Xin;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Qu, Xiaoxin
关键词：pepper veinal yellows virus; vector; non-vector; volatiles; tetra-interaction
