Transcriptome analysis of near-isogenic line provides novel insights into genes associated with panicle traits regulation in rice
文献类型： 外文期刊
作者： Zhang, Wuhan 2 ; Sun, Pingyong 1 ; He, Qiang 1 ; Shu, Fu 1 ; Deng, Huafeng 1 ;
作者机构： 1.Hunan Acad Agr Sci, Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China
2.Collaborat Innovat Ctr Grain & Oil Crops South Ch, Changsha, Hunan, Peoples R China
3.China Natl Japon Rice Res & Dev Ctr, Tianjin, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2018 年 13 卷 6 期
页码：
收录情况： SCI
摘要： Panicle traits in rice impact yield and quality. The OsGRF4 gene encodes a growth-regulating factor controlling panicle traits, and was recently cloned. Gene expression profiling analysis can be used to study the molecular mechanisms underlying OsGRF4 regulation. Use of near-isogenic lines (NILs) reduces genetic background noise in omics studies. We compared transcriptome profiling of 7 cm long young panicles of NIL-Osgrf4 and NIL-OsGRF4 using RNAs sequence analyses. Eighty differentially expressed genes (DEGs) were identified. Our target gene OsGRF4 was up-regulated in NIL-OsGRF4 plants, which is consistent with a previous qPCR analysis. Hierarchical cluster analysis showed OsGRF4 is tightly clustered with the up-regulated DEG LOC_Os02g47320. Gene Ontology (GO) and KEGG analysis suggested that DEGs were primarily involved in somatic embryogenesis and chitinase activity. Two up-regulated DEGs, LOC_Os04g41680 and LOC_Os04g41620, were significantly enriched in the top 8 GO terms, and were over_represented in term of seed development, and may play key roles in grain shape regulation. The transcription factor Osmyb1 also exhibited differential expression between NILs, and may be is an important regulator of panicle traits. By searching reported functions of DEGs and by co-localization with previous identified quantitative trait loci (QTL), we determined that the pleiotropic gene OsGRF4 may also be involve in abiotic stress resistance. This study provides new candidates genes for further understanding the molecular mechanisms underlying rice panicle trait regulation.
- 相关文献
作者其他论文 更多>>
-
Research Advances in Multiple Embryos and Apomixis in Rice (Oryza sativa L.)
作者：Dan, Junhao;Long, Wuhua;Wu, Chaoxin;Jiang, Xue;Fang, Shengyan;Long, Wuhua;Qiu, Mudan;Zhang, Longhui;Deng, Huafeng;Zhu, Susong
关键词：synthetic apomixis; hybrid rice breeding; polyembryony
-
Integrated transcriptome and BSA-seq analysis identifies a novel QTL for Meloidogyne graminicola resistance in rice HuaHang31
作者：Yang, Zhuhong;Xiao, Qingyan;Wang, Yao;Zhang, Yingjun;Ye, Shan;Ding, Zhong;Sun, Pingyong;Zhang, Wuhan;Deng, Huafeng;Liu, Shiming
关键词：
-
Phosphorylation of CPR5 by the receptor-like kinase FLR2 promotes mRNA poly(A) tail processing and immunity to Magnaporthe oryzae in rice
作者：Luo, Xiao;Xing, Junjie;Peng, Zhirong;Zhang, Min;Wei, Zhongwei;Xu, Jingbo;Deng, Huafeng;Luo, Xiao;Yu, Feng;Yu, Feng
关键词：rice; FERONIA-like receptor; plant immunity; rice blast; poly(A) tail
-
Transcriptome analysis of two photo-thermo-sensitive genic male-sterile lines reveal GA-mediated regulatory mechanism for rice panicle exsertion
作者：Fang, Pengpeng;Zeng, Jia;Deng, Huafeng;Sun, Pingyong;Fang, Pengpeng;Zhang, Wuhan;He, Qiang;Deng, Huafeng;Shu, Fu;Sun, Pingyong;Zhou, Yi;Zhang, Li;Lan, Duo;Lan, Duo
关键词：Sterile line; Panicle exsertion; Gibberellin; RNA-seq; Rice
-
Proteomes and ubiquitylomes reveal the regulation mechanism of cold tolerance mediated by OsGRF4 in rice
作者：Zhang, Li;He, Qiang;Shu, Fu;Deng, Huafeng;Zhang, Wuhan;Sun, Pingyong;Zhang, Li;Huang, Renyan;Mao, Donghai;Zeng, Jia;Fang, Pengpeng;Deng, Huafeng;Sun, Pingyong
关键词：
Oryza sativa ; cold tolerance; OsGRF4 gene; proteomic analysis; ubiquitination modification -
One-line hybrid rice with high-efficiency synthetic apomixis and near-normal fertility
作者：Dan, Junhao;Zhan, Yijie;Tian, Junyou;Deng, Huafeng;Cao, Mengliang;Xia, Yumei;Tang, Ning;Cao, Mengliang;Wang, Yao;Cao, Mengliang;Cao, Mengliang
关键词：One-line hybrid rice; Apomixis; Multiple-embryos; Atavism
-
OsJAZ4 Fine-Tunes Rice Blast Resistance and Yield Traits
作者：Zhang, Mingfeng;Luo, Xiao;Zhang, Min;Deng, Huafeng;Xing, Junjie;Zhang, Mingfeng;Luo, Xiao;Zhang, Min;Peng, Zhirong;Deng, Huafeng;Xing, Junjie;He, Wei
关键词：JAZ; jasmonic acid; rice; rice blast; gibberellin; plant immunity
