New Mechanistic Insight into the Protective Effects of Ganoderma lucidum Polysaccharides Against Palmitic Acid-Induced Cell Damage in Porcine Intestinal Epithelial Cell Line IPEC-J2
文献类型： 外文期刊
作者： Liang, Zengenni 1 ; Yuan, Zhi-Hang 2 ; Wang, Yan 3 ; Du, Zhong-Hua 4 ; Guo, Jia-Jing 1 ; Xia, Ling-Li 5 ; Shan, Yang 1 ;
作者机构： 1.Hunan Acad Agr Sci, Agr Prod Proc Inst, Changsha, Peoples R China
2.Hunan Agr Univ, Changsha, Peoples R China
3.Hunan Biol & Electromech Polytech, Changsha, Peoples R China
4.Changsha Qiantu Biol Technol Ltd Co, Changsha, Peoples R China
5.Changsha Diwei Agr Technol Ltd Co, Changsha, Peoples R China
6.Hunan Acad Agr Sci, Agr Prod Proc Inst, Changsha 410125, Peoples R China
关键词： Ganoderma lucidum polysaccharides; high-fat injury; STAT pathway; medicinal mushrooms; intestinal injury
期刊名称：NATURAL PRODUCT COMMUNICATIONS （ 影响因子：1.8； 五年影响因子：1.5 ）
ISSN： 1934-578X
年卷期： 2022 年 17 卷 11 期
页码：
收录情况： SCI
摘要： Ganoderma lucidum (G. lucidum) is one of the well-known mushrooms in China, which has G. lucidum polysaccharides (GLP) that have been widely studied for various biological activities, such as antioxidant, antitumor, antiinflammatory, antiviral, antidiabetes, and immunomodulatory activities. A signal transducer and activator of transcription (STAT) signaling pathway is related to cell proliferation and apoptosis. The relationship between STAT and intestinal protection of GLP is still unknown. We studied the inhibitors AG490 in the STAT pathway and its downstream molecules to analyze the unique effects in the protection of GLP against palmitic acid (PA)-induced porcine intestinal epithelial cells (IPEC-J2) injury. Compared to PA treatment, GLP + PA obviously decreased Ca2+ concentration, H2O2 production, NF-E2-related factor 2 (Nrf2) nuclear translocation, STAT1 and STAT2 protein levels, and increased nuclear factor kappa-B (NF-kappa B) nuclear translocation and p-STAT3/STAT3 ratio in IPEC-J2 cells. After inhibition of STAT3 signaling, p-STAT3/STAT3 ratio, NF-kappa B nuclear translocation obviously decreased and Nrf2 nuclear translocation significantly increased in the GLP + PA group. The protection of GLP on proliferation and apoptosis of PA-induced IPEC-J2 cells was suppressed by inhibiting STAT3. The STAT3 pathway regulated the enterocyte-protective effects of GLP by modulating the nuclear translocation of Nrf2 and NF-kappa B. We provide new insights into the mechanism of STAT signaling for the protection of GLP on PA-induced intestinal epithelial cell injury.
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