Selection of reliable reference genes for gene expression studies in Caenorhabditis elegans exposed to crystals (Cry1Ia36) protein of Bacillus thuringiensis
文献类型： 外文期刊
作者： Wang, Dongwei 1 ; Liu, Yong 1 ; Zhang, Deyong 1 ; He, Qingcong 1 ; Tang, Bei 1 ; Cheng, Feixue 1 ;
作者机构： 1.Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410125, Hunan, Peoples R China
2.Key Lab Integrated Management Pests & Dis Hort Cr, Changsha 410125, Hunan, Peoples R China
3.Hunan Univ, Grad Sch, Long Ping Branch, Changsha 410125, Hunan, Peoples R China
关键词： Reference genes; Quantitative real time PCR; Cry1Ia36 protein; Caenorhabditis elegans; Gene expression
期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）
ISSN： 0301-4851
年卷期： 2019 年 46 卷 6 期
页码：
收录情况： SCI
摘要： Quantitative real time PCR (qRT-PCR) is a nucleic acid quantitative technique and is also considered as a validation tool. The Cry1Ia36 protein isolated from Bacillus thuringiensis (Bt) strain YC-10 has high nematicidal activity against nematodes. Caenorhabditis elegans is one of the major model organisms and a readily accessible source of biological material for gene expression studies. To evaluate the expression stability of 12 candidate reference genes of C. elegans for exposing to different concentrations of Cry1Ia36 protein and different treat time, five statistical approaches (the comparative delta-Ct method, BestKeeper, NormFinder, Genorm and RefFinder) were used to evaluate each individual candidate reference gene. The results indicated that cdc-42 and F35G12.2 were the best reference genes for performing reliable gene expression normalization in the impact of Cry1Ia36 protein. In addition, when C. elegans was exposed to Cry1Ia36 protein and other nematicides, avermectin and 5-aminolevulinic acid, cdc-42 was recommended as the most reliable reference genes. Y45F10D.4 was the least stable reference genes in our experimental settings. Therefore, cdc-42 was reliable reference gene for gene expression studies in C. elegans exposed to Cry1Ia36 protein and other nematicides.
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