Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus
文献类型： 外文期刊
作者： Shi, Xiao-Bin 1 ; Wang, Xue-Zhong 1 ; Zhang, De-Yong 1 ; Zhang, Zhan-Hong 2 ; Zhang, Zhuo 1 ; Cheng, Ju'E 1 ; Zheng, L 1 ;
作者机构： 1.Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410000, Hunan, Peoples R China
2.Hunan Acad Agr Sci, Inst Vegetable, Changsha 410000, Hunan, Peoples R China
3.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA
关键词： Bemisia tabaci; tomato chlorotic virus; volatile organic compounds; odor binding proteins; RNA interference; feeding preference
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2019 年 20 卷 20 期
页码：
收录情况： SCI
摘要： Tomato chlorosis virus (ToCV) is widespread, seriously impacting tomato production throughout the world. ToCV is semi-persistently transmitted by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Currently, insect olfaction is being studied to develop novel pest control technologies to effectively control B. tabaci and whitefly-borne virus diseases. Despite current research efforts, no report has been published on the role of odorant-binding proteins (OBPs) in insect preference under the influence of plant virus. Our previous research showed that viruliferous B. tabaci preferred healthy plants at 48 h after virus acquisition. In this study, we determined the effect of OBPs on the host preference interactions of ToCV and whiteflies. Our results show that with the increase in acquisition time, the OBP gene expressions changed differently, and the OBP3 gene expression showed a trend of first rising and then falling, and reached the maximum at 48 h. These results indicate that OBP3 may participate in the host preference of viruliferous whiteflies to healthy plants. When the expression of the OBP3 gene was knocked down by an RNA interference (RNAi) technique, viruliferous Mediterranean (MED) showed no preference and the ToCV transmission rate was reduced by 83.3%. We conclude that OBP3 is involved in the detection of plant volatiles by viruliferous MED. Our results provide a theoretical basis and technical support for clarifying the transmission mechanism of ToCV by B. tabaci and could provide new avenues for controlling this plant virus and its vectors.
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