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Mapping and identifying candidate genes involved in the novel fasciculate inflorescence in pepper (Capsicum annuum L.)

文献类型： 外文期刊

作者： Lv, Junheng 1 ; Liu, Yuhua 1 ; Liu, Zhoubin 1 ; Wang, Jing 1 ; Ma, Yanqing 2 ; Zhang, Zhuqing 2 ; Yang, Sha 2 ; Ou, Lijun 3 ;

作者机构： 1.Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Hunan, Peoples R China

2.Hunan Acad Agr Sci, Vegetable Inst, Changsha 410125, Hunan, Peoples R China

3.Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China

关键词： Bulked segregant analysis; Linkage mapping; Fasciculate inflorescence; Capsicum annuum L

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2019 年 39 卷 11 期

页码：

收录情况： SCI

摘要： As a key determinant affecting yield and quality in pepper, inflorescence architecture is a significant feature of the ornamental plants. In our present study, a new mutant with fasciculate inflorescence named CL74 was derived from our germplasm resources. Genetic analysis of CL74 indicated that it was different from the fasciculate (fa) mutant, and its fasciculate inflorescence trait was controlled by a single recessive nuclear gene. A large F-2 population was constructed and used for bulked segregant analysis (BSA) and linkage analysis. These findings revealed that the candidate gene was mapped to a segment with a physical distance of approximately 998 kb between Indel-197064077 and Indel-198110136. There were two agamous-like MADS-box protein genes Capanan11g001832 and Capanan11g001834 in the candidate region. Besides, two single nucleotide polymorphisms (SNPs) existed on the coding region of Capanan11g001832 in the CL74 mutant compared with the single-flower plants. In addition, the two SNPs were verified and fully co-segregated with fasciculate inflorescence phenotype in the F-2 individuals. Collectively, Capanan11g001832 was a strong candidate gene for the fasciculate inflorescence. Furthermore, transcriptome analysis indicated that three WUSCHEL-like genes (Capana00g000667, Capana06g000476, and Capana11g000671) were downregulated and CLV1 (Capana04g000175) was upregulated in CL74. Moreover, we speculated that there was a complex relationship among the genes Capanan11g001832, Capanan11g001834, the three WUSCHEL-like genes, and CLV1, and these genes might co-regulate the formation of fasciculate inflorescence in pepper. Our study laid the foundation for the selection of high-yield, mechanized harvesting, or ornamental varieties of pepper.

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