A genetic tool for production of GFP-expressing Rhodopseudomonas palustris for visualization of bacterial colonization
文献类型： 外文期刊
作者： Zhai, Zhongying 1 ; Du, Jiao 2 ; Chen, Lijie 1 ; Hamid, Muhammad Rizwan 2 ; Du, Xiaohua 2 ; Kong, Xiaoting 1 ; Cheng, 1 ;
作者机构： 1.Hunan Univ, Grad Sch, Longping Branch, Changsha 410125, Hunan, Peoples R China
2.Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China
关键词： Rhodopseudomonas palustris; Green fluorescent protein; Colonization; CLSM; Visualization
期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）
ISSN： 2191-0855
年卷期： 2019 年 9 卷 1 期
页码：
收录情况： SCI
摘要： Development of a genetic tool for visualization of photosynthetic bacteria (PSB) is essential for understanding microbial function during their interaction with plant and microflora. In this study, Rhodopseudomonas palustris GJ-22-gfp harboring the vector pBBR1-pckA(PT)-gfp was constructed using an electroporation transformation method and was used for dynamic tracing of bacteria in plants. The results showed that strain GJ-22-gfp was stable and did not affect the biocontrol function, and the Confocal Laser Scanning Microscopy (CLSM) results indicated it could successfully colonised on the surface of leaf and root of tobacco and rice. In tobacco leaves, cells formed aggregates on the mesophyll epidermal cells. While in rice, no aggregate was found. Instead, the fluorescent cells colonise the longitudinal intercellular spaces between epidermal cells. In addition, the results of strain GJ-22 on the growth promotion and disease resistance of tobacco and rice indicated that the different colonization patterns might be related to the bacteria could induce systemic resistance in tobacco.
- 相关文献
作者其他论文 更多>>
-
Endophytic Bacterial Community Structure and Function Response of BLB Rice Leaves After Foliar Application of Cu-Ag Nanoparticles
作者：Ning, Weimin;Li, Mingxuan;Jiang, Lei;Yang, Mei;Liu, Maoyan;Ning, Weimin;Liu, Yong;Liu, Yong
关键词：endophytic bacteria; Cu-Ag nanoparticles; bacterial community; BLB; rice
-
Cucumber green mottle mosaic virus encodes additional small proteins with specific subcellular localizations and virulence function
作者：Gong, Pan;Gao, Mengxin;Chen, Yalin;Zhang, Mingzhen;Hu, Xiaohua;Zhao, Siwen;Pan, Mengjiao;Cao, Buwei;Zhou, Xueping;Li, Fangfang;Gao, Mengxin;Zhou, Xueping;Huang, Yucong;Shen, Qingtang;Zhang, Hui;Liu, Yong;Liu, Yong;Lozano-Duran, Rosa;Wang, Aiming
关键词：positive-sense single-stranded RNA viruses; tobamoviruses; cucumber green mottle mosaic virus; small ORFs; peroxisomes; virus pathogenicity
-
Host metabolites explain microbiome variation between different rice genotypes
作者：Su, Pin;Peng, Weiye;Sun, Shu'e;Du, Xiaohua;Bai, Lianyang;Liu, Yong;Zhang, Deyong;Su, Pin;Peng, Weiye;Sun, Shu'e;Du, Xiaohua;Bai, Lianyang;Liu, Yong;Zhang, Deyong;Kang, Houxiang;Peng, Qianze;Zhang, Deyong;Peng, Qianze;Zhang, Deyong;Zhang, Chi;Lei, Ziling;Liu, Yong;Zhang, Deyong;Tang, Qianjun;Cernava, Tomislav
关键词：Plant genotype; Plant microbiome; Genetic control; Plant metabolites; Phenylpropanoids
-
Analysis of the Antagonistic Effect of Dibutyl Phthalate on Magnaporthe oryzae through Bioassay and Omics Data
作者：Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue;Qin, Yingfei;Wu, Xiyang;Tan, Xinqiu;Liu, Yong;Zhang, Deyong;Chen, Yue
关键词：dibutyl phthalate; antifungal activity; Magnaportheoryzae; transcriptome; metabolome
-
Metabolomic Profiling Reveals the Effects of Cu-Ag Nanoparticles on Tomato Bacterial Wilt
作者：Ning, Weimin;Jiang, Lei;Yang, Mei;Lei, Tianhao;Liu, Chan;Zhao, Fei;Shu, Pan;Ning, Weimin;Liu, Yong;Ning, Weimin;Liu, Yong
关键词：Cu-Ag nanoparticles; tomato; metabolomics; fatty acyls; organooxygen compounds
-
Transcriptome Analysis Reveals the Role of OsCBM1 in Rice Defense Against Xanthomonas oryzae pv.oryzae
作者：Bie, Shuaijun;Liu, Yong;Zhang, Deyong;Xiao, Youlun;Liu, Yong;Peng, Jing;Gao, Yang;Li, Xiaojuan;Tan, Xinqiu;Huang, Renyan;Zhang, Deyong;Zhang, Li;He, Xiaomin;Xie, Hongjun;Huang, Renyan;Zhang, Deyong
关键词：malectin-like domain-containing protein;
OsCBM1 ;Xanthomonas oryzae pv. oryzae ; reactive oxygen species; phytohormones; rice -
A viral movement protein mediates host volatile biosynthesis to co-attract vectors and non-vectors and enhances viral infection
作者：Li, Shijun;Luo, Xiangwen;Zhang, Zhanhong;Ouyang, Xian;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Luo, Xiangwen;Zhang, Yu;Zhang, Zhanhong;Yang, Xiao;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Luo, Xiangwen;Zhang, Yu;Zhang, Zhanhong;Yang, Xiao;Ouyang, Xian;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Zhou, Xuguo;Wang, Xin;Zhang, Deyong;Zhang, Songbai;Liu, Yong;Qu, Xiaoxin
关键词：pepper veinal yellows virus; vector; non-vector; volatiles; tetra-interaction
