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Targeted mutations of BnPAP2 lead to a yellow seed coat in Brassica napus L.

文献类型： 外文期刊

作者： Huang, Wei 1 ; Jiao, Ruyu 2 ; Cheng, Hongtao 3 ; Cai, Shengli 3 ; Liu, Jia 3 ; Hu, Qiong 3 ; Liu, Lili 1 ; Li, Bao 1 ; Wang, Tonghua 1 ; Li, Mei 1 ; Zhang, Dawei 2 ; Yan, Mingli 1 ;

作者机构： 1.Hunan Acad Agr Sci, Crop Res Inst, Hunan Res Ctr Heterosis Utilizat Rapeseed, Changsha 410125, Peoples R China

2.Hunan Univ Sci & Technol, Sch Life & Hlth Sci, Hunan Key Lab Econ Crops Genet Improvement & Integ, Xiangtan 411201, Peoples R China

3.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs, Wuhan 430062, Peoples R China

关键词： yellow seed; BnPAP2; proanthocyanidins; CRISPR/Cas9

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）

ISSN： 2095-3119

年卷期： 2024 年 23 卷 2 期

页码：

收录情况： SCI

摘要： The yellow seed trait is preferred by breeders for its potential to improve the seed quality and commercial value of Brassica napus. In the present study, we produced yellow seed mutants using a CRISPR/Cas9 system when the two BnPAP2 homologs were knocked out. Histochemical staining of the seed coat demonstrated that proanthocyanidin accumulation was significantly reduced in the pap2 double mutants and decreased specifically in the endothelial and palisade layer cells of the seed coat. Transcriptomic and metabolite profiling analysis suggested that disruption of the BnPAP2 genes could reduce the expression of structural and regulated genes in the phenylpropanoid and flavonoid biosynthetic pathways. The broad suppression of these genes might hinder proanthocyanidin accumulation during seed development, and thereby causing the yellow seed trait in B. napus. These results indicate that BnPAP2 might play a vital role in the regulatory network controlling proanthocyanidin accumulation.

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