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Down-regulation of 14-3-3 genes suppress sugar metabolism in Bemisia tabaci MED and reduce transmission of tomato chlorosis virus

文献类型： 外文期刊

作者： Wei, Huanwen 1 ; Huang, Liping 2 ; Wang, Pei 2 ; Zhang, Zhanhong 3 ; Chen, Jianbin 2 ; Du, Jiao 2 ; Yan, Shuo 2 ; Zhang, Deyong 1 ; Shi, Xiaobin 1 ; Liu, Yong 1 ;

作者机构： 1.Hunan Univ, Coll Biol, Longping Branch, Grad Sch, Changsha, Peoples R China

2.Hunan Acad Agr Sci, Inst Plant Protect, 726 2nd Sect Yuanda Rd, Changsha 410125, Peoples R China

3.Yuelushan Lab, Changsha, Peoples R China

4.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Yu, Kunming, Peoples R China

5.Hunan Acad Agr Sci, Inst Vegetable, Changsha, Peoples R China

关键词： Bemisia tabaci MED; tomato chlorosis virus; 14-3-3 protein; RNA interference; sugar metabolism

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）

ISSN： 1526-498X

年卷期： 2025 年 81 卷 9 期

页码：

收录情况： SCI

摘要： BACKGROUNDTomato chlorosis virus (ToCV) is an RNA virus that seriously reduces tomato yield, mainly transmitted by whiteflies (Bemisia tabaci) in a semi-persistent manner. Currently, preventing ToCV transmission by selecting the target gene of whiteflies is an effective measure to control ToCV. Proteomic analysis revealed that 14-3-3 protein levels significantly increase in ToCV-infected B. tabaci. To verify the target role of 14-3-3 protein, its gene expression was down-regulated with RNA interference (RNAi) technology to explore its function in ToCV acquisition and transmission, and to find a new strategy for controlling ToCV transmission by whiteflies.RESULTSThe amount of ToCV was positively correlated with the expression of Bt14-3-3 epsilon and Bt14-3-3 zeta in B. tabaci, and ToCV promotes glycogen synthesis via the phosphoinositide-3-kinase/protein kinase B pathway (PI3K/AKT pathway). After silencing Bt14-3-3 epsilon and Bt14-3-3 zeta, ToCV acquisition, transmission, oviposition and sugar metabolism of B. tabaci were suppressed.CONCLUSIONSThese results illustrated that ToCV induced Bt14-3-3 epsilon and Bt14-3-3 zeta, and promoted glycogen synthesis via PI3K/AKT pathway in B. tabaci. Silencing 14-3-3 genes prevented ToCV acquisition, ToCV transmission, oviposition and sugar metabolism of B. tabaci. It could provide a basis for establishing a new strategy to regulate ToCV transmission of B. tabaci by using 14-3-3 protein. (c) 2025 Society of Chemical Industry.

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