文献类型： 外文期刊

作者： Guo, Tianyi ¹ ; Zhu, Lingfeng ¹ ; Zhou, Yaping ¹ ; Han, Shuai ¹ ; Cao, Yunyun ¹ ; Hu, Zuomin ¹ ; Luo, Yi ³ ; Bao, Liyuan ⁴ ; Wu, Xiuxiu ¹ ; Qin, Dandan ¹ ; Lin, Qinlu ¹ ; Luo, Feijun ¹ ;

作者机构： 1.Cent South Univ Forestry & Technol, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Hunan Key Lab Forestry Edible Resources Safety & P, Changsha, Peoples R China

2.Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Changsha, Peoples R China

3.Cent South Univ, Xiangya Sch Med, Dept Clin Med, Changsha, Peoples R China

4.Changsha Univ, Dept logist, Changsha, Peoples R China

5.Cent South Univ Forestry & Technol, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Hunan Key Lab Forestry Edible Resources Safety & P, Changsha 410004, Hunan, Peoples R China

关键词： alcoholic liver disease; laminarin; molecular mechanisms; pathways

期刊名称：JOURNAL OF FOOD BIOCHEMISTRY （ 影响因子：3.654； 五年影响因子：3.271 ）

ISSN： 0145-8884

年卷期：

页码：

收录情况： SCI

摘要： Alcoholic liver disease (ALD) has become a health issue globally. Laminarin, a low molecular weight marine-derived beta-glucan, has been identified with multiple biological activities. In this study, the ameliorative effect on ALD of laminarin isolated from brown algae was investigated. Phenotypic, pathological alterations and biochemical characteristics indicated that laminarin administration (100 mg/kg/day) significantly alleviated liver injury and improved liver function in the alcohol-induced mice. Gene chip results indicated that laminarin treatment caused 52 up-regulated and 13 down-regulated genes in the hepatic tissues of alcohol-induced damage mice, and most of these genes are associated with regulation of oxidative stress (such as CYP450/glutathione-dependent antioxidation), Wnt signaling pathway, retinol metabolism, and cAMP pathway based on GO and KEGG analysis. PPI network analysis indicated that the downstream target genes lied in the hub of the net. Our experiments also confirmed the changed expressions of some target genes. Taken together, these results suggest that laminarin can ameliorate alcohol-induced damage in mice and its molecular mechanism lies in modulating anti-oxidation pathway, WNT pathway, and cAMP pathway, which regulate the expressions of downstream target genes and alleviate alcohol-induced damage. Our study provides new clue to prevent alcohol-induced damage and will be benefit to develop functional foods. Practical applicationsThis study verified the positive effect on alcoholic liver disease (ALD) of laminarin, a water-soluble brown algae-derived beta-glucan, linked by beta-(1,3) glycosidic bonds with beta-(1,6) branches. Laminarin significantly alleviated liver injury and improved liver function of ALD mice. Moreover, transcriptomics and bioinformatics analysis further revealed the gene expression patterns, hub targets, and signalings including CYP450/glutathione, Wnt, retinol metabolism, cAMP pathways regulated by laminarin. This research is the first evidence for hepatoprotective effect of laminarin against ALD and its molecular mechanism, which will be advantage to develop functional foods or adjuvant therapy of ALD.