文献类型： 外文期刊
作者： Chen, Lihong 1 ; Zhou, Junfei 1 ; Li, Tiantian 1 ; Fang, Zhiwei 1 ; Li, Lun 1 ; Huang, Gang 1 ; Gao, Lifen 1 ; Zhu, Xiaob 1 ;
作者机构： 1.Jianghan Univ, Inst Syst Biol, Wuhan 430056, Hubei, Peoples R China
2.Wuhan Qingfahesheng Seed Co Ltd, Wuhan 430056, Hubei, Peoples R China
3.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China
4.Mingliao Biotechnol Co Ltd, Wuhan 430056, Peoples R China
5.Beijing Technol & Business Univ, Sch Food & Hlth, Beijing 100048, Peoples R China
6.Washington Univ, Dept Genet, Dept Comp Sci & Engn, St Louis, MO 63130 USA
7.MolBreeding Biotechnol Co Ltd, Shijiazhuang 050035, Hebei, Peoples R China
8.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
关键词： GmoDetector; GMO identification; GMO characteristic fragment; Probe capture; Next generation sequencing
期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：6.475； 五年影响因子：6.508 ）
ISSN： 0963-9969
年卷期： 2021 年 149 卷
页码：
收录情况： SCI
摘要： The rapid increase of genetically modified organisms (GMOs) entering the food and feed markets, and the contamination of donor (micro)organisms of transgenic elements make it more challenging for the existing GMO detection. In this study, we developed a high-throughput and contamination-removal GMO detection approach named as GmoDetector. GmoDetector targeted 64 common transgenic elements and 76 GMO-specific events collected from 251 singular GM events, and combined with next generation sequencing (NGS) and target enrichment technology to detect various GMOs. As a result, GmoDetector was able to exclude the donor (micro) organism contamination, and detect the authorized and unauthorized GMOs (UGMOs) in any forms of food or feed, such as processed or unprocessed. The sensitivity of GmoDetector is as low as 0.1% (GMO content), which has met the GMO labeling threshold for all countries. Therefore, GmoDetector is a robust tool for accurate and efficient detection of the authorized and UGMOs.
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