文献类型： 会议论文
第一作者： Zhan Qingcai
作者： Zhan Qingcai 1 ; Liu Zhixi 1 ; Chen Zuwu 1 ; Zhu Keyong 1 ; Li Zhaohua 1 ; Xiao Zifa 1 ;
作者机构： 1.Laboratory of Biotechnology, Rice Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China
关键词： hybrid rice;simple sequence repeats;purity identification;technology optimization
会议名称： International symposium on hybrid rice
主办单位：
页码： 1-6
摘要： To overcome factors limiting the commercialization of a rapid identification system for hybrid seed purity by using simple sequence repeats (SSRs), this report offers an improved technology for DNA extraction, the PCR amplification process, and gel electrophoresis. For example, the time for DNA extraction,PCR amplification, and gel electrophoresis has been decreased to 2.5 min, 65 min, and 1 h, respectively. Meanwhile, we have reduced the cost, decreased the dosage of dNTP and AmpliTaq Gold DNA enzyme, reused the agarose gel, and abated environmental pollution. Therefore, an accurate, simple, rapid, and low-cost identification system for hybrid seed purity has been established.
分类号： Q781`S334
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