Effect of Nitrogen Form on Gene Expression in Leaf Tissue of Greenhouse Grown Potatoes During Three Stages of Growth
文献类型： 外文期刊
作者： Zebarth, Bernie J. 1 ; Tai, Helen 1 ; Luo, Sainan 2 ; Millard, Pete 3 ; De Koeyer, David 1 ; Li, Xiu-Qing 1 ; Xiong, X 1 ;
作者机构： 1.Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada
2.Hunan Acad Agr Sci, Inst Hort, Changsha 410025, Hunan, Peoples R China
3.Craigiebuckler, James Hutton Inst, Aberdeen AB15 8QH, Scotland
4.Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China
关键词： Nitrate;Ammonium;Nitrate transporter;Ammonium transporter;Potato;Solanum tuberosum
期刊名称：AMERICAN JOURNAL OF POTATO RESEARCH （ 影响因子：1.697； 五年影响因子：1.673 ）
ISSN： 1099-209X
年卷期： 2012 年 89 卷 4 期
页码：
收录情况： SCI
摘要： This study examined the expression of genes in leaf tissue of greenhouse-grown potatoes to the form of N supplied in the context of development of a diagnostic tool for detection of N status. Potato cultivar 'Shepody' was grown over a two-week period at three stages of growth (tuber initiation, flowering and early tuber bulking) with three forms of N (NO3(-) only, NH4(+) only, or 1:1 NO3(-):NH4(+)) at two levels of N supply (0.75 or 7.5 mM). Leaf disks were collected and extracted for RNA, and 28 genes examined for expression using nCounter. Expression of ammonium transporter AT1 was increased when the N supply was reduced, regardless of N form, and was generally independent of growth stage. Expression of AT1 was less influenced by N form than currently used chemical or optical measures of plant N status. Expression of AT1 can be used as a quantitative indicator for plant N status of potato regardless of nitrogen form and crop growth stage.
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