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Isolation and characterization of a novel 2-methyl-4-chlorophenoxyacetic acid-degrading Enterobacter sp strain SE08

文献类型： 外文期刊

作者： Tan, Lin 2 ; Hu, Qiulong 1 ; Xiong, Xingyao 1 ; Su, Xiaojun 1 ; Huang, Yanning; Jiang, Ziwei 1 ; Zhou, Qingming 1 ; Zh 1 ;

作者机构： 1.Hunan Agr Univ, Changsha 410128, Hunan, Peoples R China

2.Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China

3.Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China

4.Chenzhou Tobacco Co Hunan Prov, Chenzhou 423000, Hunan, Peoples R China

5.Tobacco Co Hunan Prov, Changsha 410007, Hunan, Peoples R China

6.Tobacco Co Hunan Prov, Changsha 4100

关键词： 2-Methyl-4-chlorophenoxy acetic acid;Enterobacter;Degradation;Bacterial growth

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）

ISSN： 0147-6513

年卷期： 2013 年 96 卷

页码：

收录情况： SCI

摘要： A bacterial strain (SE08) capable of utilizing 2-methyl-4-chlorophenoxy acetic acid (MCPA) as the sole carbon and energy source for growth was isolated by continuous enrichment culturing in minimal salt medium (MSM) from a long term MCPA exposed soil. This bacterial strain was identified as Enterobacter sp. based on morphological, physiological and biochemical tests, as well as 16S rRNA sequence analysis. Its ability to degrade MCPA was determined using high performance liquid chromatography. The strain SE08 can tolerate unusually high MCPA concentrations (125-2000 mg/L). The influences of culturing factors (initial concentration, pH, and temperature) on the bacterial growth and substrate degradation were studied. The results showed that the optimal MCPA degradation occurred at an MCPA concentration of 500 mg/L, 30 degrees C and pH 6.0. Under these conditions, 68.5 percent of MCPA in MSM was degraded by SE08, and the OD600 (nm) reached 0.64 after culturing for 72 h. The degradation of MCPA could be enhanced by addition of both carbon and nitrogen sources. At an initial MCPA concentration of 500 mg/L, when 5 g/L glucose and 2.5 g/L yeast extract were added into the MSM media, the MCPA degradation was significantly increased to 83.8 percent, and OD600 (nm) was increased to 1.09 after incubation at 30 degrees C and pH 6.0 for 72 h. This is the first study showing that an Enterobacter sp. strain is capable of degrading MCPA, which might provide a new approach for the remediation of MCPA contaminated soil and contribute to the limited knowledge about the function of Enterobacter species. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.

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