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Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.)

文献类型： 外文期刊

作者： Yang, Qian 1 ; Deng, Wei 1 ; Li, Xuefeng 1 ; Yu, Qin 2 ; Bai, Lianyang 3 ; Zheng, Mingqi 1 ;

作者机构： 1.China Agr Univ, Coll Sci, Dept Appl Chem, Beijing 100193, Peoples R China

2.Univ Western Australia, Sch Plant Biol, Australian Herbicide Resistance Initiat, Crawley, WA 6009, Australia

3.Hunan Acad Agr Sci, Changsha 410125, Hunan, Peoples R China

关键词： Acetolactate synthase;Cytochrome P450;Flixweed;Tribenuron-methyl;Target-site based resistance;Non-target-site based resistance;Metabolic resistance;RNA-Seq

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2016 年 17 卷

页码：

收录情况： SCI

摘要： Background: Flixweed (Descurainia sophia L.) is a troublesome and widespread broadleaf weed in winter fields in China, and has evolved high level resistance to acetolactate synthase (ALS)-inhibiting sulfonylurea herbicide tribenuron-methyl. Results: We identified a resistant flixweed population (N11) exhibiting 116.3-fold resistance to tribenuron-methyl relative to the susceptible population (SD8). Target-site ALS gene mutation Pro-197-Thr was identified in resistant plants. Moreover, the resistance can be reversed to 28.7-fold by the cytochrome P450 inhibitor malathion. The RNA-Sequencing was employed to identify candidate genes involved in non-target-site metabolic resistance in this population. Total 26 differentially expressed contigs were identified and eight of them (four P450s, one ABC transporter, three glycosyltransferase) verified by qRT-PCR. Consistent over-expression of the two contigs homology to CYP96A13 and ABCC1 transporter, respectively, were further qRT-PCR validated using additional plants from the resistant and susceptible populations. Conclusions: Tribenuron-methyl resistance in flixweed is controlled by target-site ALS mutation and non-target-site based mechanisms. Two genes, CYP96A13 and ABCC1 transporter, could play an important role in metabolic resistance to tribenuron-methyl in the resistant flixweed population and justify further functional studies.

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