Transcriptomic analyses reveal variegation-induced metabolic changes leading to high L-theanine levels in albino sectors of variegated tea (Camellia sinensis)
文献类型： 外文期刊
作者： Xie, Nianci 1 ; Zhang, Chenyu 1 ; Zhou, Pinqian 3 ; Gao, Xizhi 1 ; Wang, Minghan 1 ; Tian, Shuanghong 4 ; Lu, Cui 1 ; Wang, Kunbo 1 ; Shen, Chengwen 1 ;
作者机构： 1.Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China
2.Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China
3.Hunan Acad Agr Sci, Tea Res Inst, Changsha 410125, Hunan, Peoples R China
4.Xiangxi Acad Agr Sci, Jishou, Jishou 416000, Hunan, Peoples R China
关键词： Camellia sinensis; Transcriptome; Variegation; Theanine; Chlorophyll synthesis
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.437； 五年影响因子：5.731 ）
ISSN： 0981-9428
年卷期： 2021 年 169 卷
页码：
收录情况： SCI
摘要： Camellia sinensis cv. 'Yanling Huayecha' (YHC) is an albino-green chimaeric tea mutant with stable genetic traits. Here, we analysed the cell ultrastructure, photosynthetic pigments, amino acids, and transcriptomes of the albino, mosaic, and green zones of YHC. Well-organized thylakoids were found in chloroplasts in mesophyll cells of the green zone but not the albino zone. The albino zone of the leaves contained almost no photosynthetic pigment. However, the levels of total amino acids and theanine were higher in the albino zone than in the mosaic and green zones. A transcriptomic analysis showed that carbon metabolism, nitrogen metabolism and amino acid biosynthesis showed differences among the different zones. Metabolite and transcriptomic analyses revealed that (1) downregulation of CsPPOX1 and damage to thylakoids in the albino zone may block chlorophyll synthesis; (2) downregulation of CsLHCB6, CsFdC2 and CsSCY1 influences chloroplast biogenesis and thylakoid membrane formation, which may contribute to the appearance of variegated tea leaves; and (3) tea plant variegation disrupts the balance between carbon and nitrogen metabolism and promotes the accumulation of amino acids, and upregulation of CsTSI and CsAlaDC may enhance L-theanine synthesis. In summary, our study provides a theoretical basis and valuable insights for elucidating the molecular mechanisms and promoting the economic utilization of variegation in tea.
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