A high-throughput screening method for endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice (Oryza sativa L.) seeds
文献类型： 外文期刊
作者： Wang, Zhishan 1 ; Wu, Xianyu 1 ; Li, Ni 2 ; Wang, Weiping 2 ; Liu, Yang 1 ;
作者机构： 1.Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Beijing 100083, Peoples R China
2.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China
关键词： Rice seed; Endophytes; High-throughput screening; Antifungal activity; Biological control
期刊名称：PLANT GROWTH REGULATION （ 影响因子：3.412； 五年影响因子：3.691 ）
ISSN： 0167-6903
年卷期：
页码：
收录情况： SCI
摘要： Screening for microorganisms with antagonistic activity against crop pathogens is essential for microbial antagonist preparation, crop disease control, and sustainable agricultural development. This study established a rapid, efficient, one-step high-throughput method for screening endophytic bacteria displaying antagonistic activity against Magnaporthe oryzae (M. oryzae) ACCC36020 in rice seeds. The experimental results indicated that these bacteria could be directly screened from six sample groups using this method. This technique can achieve simultaneous screening and purification, identify endophytic bacteria displaying antifungal activity in rice seeds, and save significant time.
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