N318L Blocks the Interaction of Fluralaner but Not Broflanilide or Fipronil with the Insect GABA Receptor In Vivo
文献类型： 外文期刊
作者： Zhang, Yichi 1 ; Liu, Xinyu 1 ; Wang, Junyan 1 ; Wang, Ying 1 ; Amponsah, Priscilla 1 ; Tang, Tao 2 ; Jones, Andrew K. 3 ; Zhao, Chunqing 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Pest Management Crops East Chin, Key Lab Integrated Management Crop Dis & Pests,Min, Nanjing 210095, Peoples R China
2.Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410125, Peoples R China
3.Oxford Brookes Univ, Dept Biol & Med Sci, Oxford OX3 0BP, England
关键词： chloride channel; resistanceto dieldrin; isoxazolines; Drosophila; CRISPR/Cas9
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 41 期
页码：
收录情况： SCI
摘要： Fluralaner is a novel insecticide targeting the ionotropic GABA receptor (GABAR) subunit, RDL. A recent study revealed that N316L, a substitution of asparagine (N) with leucine (L), in the second transmembrane (M2)-spanning region reduced the antagonist action of fluralaner on the housefly Musca domestica RDL (MdRDL) in vitro. To verify the impact of N316L in vivo, the corresponding mutation (N318L) in the fruitfly Drosophila melanogaster RDL (DmRDL) was constructed using CRISPR/Cas9 genome editing. The homozygous DmRDL(N318L) mutant showed a 9.87-fold resistance to fluralaner compared with w(1118) while still being highly sensitive to broflanilide and fipronil, which is consistent with those findings observed in the electrophysiology assays of the homomeric DmRDL(WT) or DmRDL(N318L) channel. Moreover, DmRDL(N318L) led to malformed ovaries, stunted eggs, and sterility in homozygous females. These results highlighted N318 as a molecular site for fluralaner in vivo and in vitro and might elucidate the resistance mechanisms of insects against fluralaner.
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