文献类型： 外文期刊

作者： Guo Xia-Yu ¹ ; Zhang Meng ⁴ ; Zhu Ming-Dong ⁵ ; Long Ji-Rui ³ ; Wei Zhong-Wei ³ ; Li Jian-Wu ³ ; Zhou Bin ⁵ ; Ai Zhi-Yong ² ; Deng Hua-Feng ¹ ;

作者机构： 1.Hunan Agr Univ, Coll Agron, Changsha 410125, Peoples R China

2.Natl Innovat Ctr Saline Alkali Tolerant Rice Sany, Sanya 572000, Peoples R China

3.Hunan Hybrid Rice Res Ctr, Changsha 410125, Peoples R China

4.Hunan Univ, Coll Biol, Hunan Key Lab Plant Funct Genom & Dev Regulat, Changsha 410082, Hunan, Peoples R China

5.Hunan Rice Res Inst, Key Lab Ind Rice Genet & Breeding Middle & Lower, Changsha 410125, Peoples R China

6.Hunan Acad Agr Sci, Changsha 410125, Peoples R China

关键词： Super hybrid rice; CY1000; Salt tolerance; Molecular mechanism; Ubiquitination

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.26； 五年影响因子：5.761 ）

ISSN： 1471-2229

年卷期： 2022 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background Soil salinization is a threat to food security. China is rich in saline land resources for potential and current utilization. The cultivation and promotion of salt-tolerant rice varieties can greatly improve the utilization of this saline land. The super hybrid rice Chaoyouqianhao (CY1000) is one of the most salt-tolerant rice varieties and is widely used, but the molecular mechanism underlying its salt tolerance is not clear. Results In this study, the characteristics of CY1000 and its parents were evaluated in the field and laboratory. The results showed that aboveground parts of CY1000 were barely influenced by salt stress, while the roots were less affected than those of its parents. A comparative transcriptomic strategy was used to analyze the differences in the response to salt stress among the male and female parents of CY1000 at the seedling stage and the model indica rice 93-11. We found that the salt tolerance of CY1000 was mainly inherited from its male parent R900, and its female parent GX24S showed hardly any salt tolerance. To adapt to salt stress, CY1000 and R900 upregulated the expression of genes associated with soluble component synthesis and cell wall synthesis and other related genes and downregulated the expression of most genes related to growth material acquisition and consumption. In CY1000 and R900, the expression of genes encoding some novel key proteins in the ubiquitination pathway was significantly upregulated. After treatment with MG-132, the salt tolerance of CY1000 and R900 was significantly decreased and was almost the same as that of the wild type after salt stress treatment, indicating that ubiquitination played an important role in the salt tolerance mechanism of CY1000. At the same time, we found that some transcription factors were also involved in the salt stress response, with some transcription factors responding only in hybrid CY1000, suggesting that salt tolerance heterosis might be regulated by transcription factors in rice. Conclusion Our results revealed that the ubiquitination pathway is important for salt tolerance in rice, and several novel candidate genes were identified to reveal a novel salt tolerance regulation network. Additionally, our work will help clarify the mechanism of heterosis in rice. Further exploration of the molecular mechanism underlying the salt tolerance of CY1000 can provide a theoretical basis for breeding new salt-tolerant rice varieties.