您好，欢迎访问湖南省农业科学院 机构知识库！

The introgression of BjMYB113 from Brassica juncea leads to purple leaf trait in Brassica napus

文献类型： 外文期刊

作者： Zhang, Dawei 1 ; Zhou, Hongfeng 1 ; Zhou, Dinggang 1 ; Wu, Jinfeng 1 ; Liu, Lili 1 ; Guo, Yiming 2 ; Wang, Tonghua 2 ; Tan, Chen 4 ; Chen, Daozong 4 ; Ge, Xianhong 5 ; Yan, Mingli 2 ;

作者机构： 1.Hunan Univ Sci & Technol, Sch Life Hlth Sci, Hunan Key Lab Econ Crops Genet Improvement & Integ, Xiangtan 411201, Peoples R China

2.Yuelushan Lab, Changsha 410128, Peoples R China

3.Hunan Acad Agr Sci, Crop Res Inst, Hunan Res Ctr Heterosis Utilizat Rapeseed, Changsha 410125, Peoples R China

4.Gannan Normal Univ, Coll Life Sci, Ganzhou Key Lab Greenhouse Vegetable, Ganzhou 341000, Peoples R China

5.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China

关键词： Brassica; Leaf color; Map-based cloning; BjMYB113; CRISPR/Cas9; Anthocyanin accumulation

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.3； 五年影响因子：5.2 ）

ISSN： 1471-2229

年卷期： 2024 年 24 卷 1 期

页码：

收录情况： SCI

摘要： The purple leaves of Brassica napus are abundant in anthocyanins, which are renowned for their role in conferring distinct colors, stress tolerance, and health benefits, however the genetic basis of this trait in B. napus remains largely unelucidated. Herein, the purple leaf B. napus (PL) exhibited purple pigments in the upper epidermis and a substantial increase in anthocyanin accumulation, particularly of cyanidin, compared to green leaf B. napus (GL). The genetic control of the purple leaf trait was attributed to a semi-dominant gene, pl, which was mapped to the end of chromosome A03. However, sequencing of the fragments amplified by the markers linked to pl indicated that they were all mapped to chromosome B05 from B. juncea. Within this B05 chromosomal segment, the BjMYB113 gene-specific marker showed perfect co-segregation with the purple leaf trait in the F-2 population, suggesting that the BjMYB113 introgression from B. juncea was the candidate gene for the purple leaf trait in B. napus. To further verify the function of candidate gene, CRISPR/Cas9 was performed to knock out the BjMYB113 gene in PL. The three myb113 mutants exhibited evident green leaf phenotype, absence of purple pigments in the adaxial epidermis, and a significantly reduced accumulation of anthocyanin compared to PL. Additionally, the genes involved in positive regulatory (TT8), late anthocyanin biosynthesis (DFR, ANS, UFGT), as well as transport genes (TT19) were significantly suppressed in the myb113 mutants, further confirming that BjMYB113 was response for the anthocyanin accumulation in purple leaf B. napus. This study contributes to an advanced understanding of the regulation mechanism of anthocyanin accumulation in B. napus.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台