Characterization ofPtAOS1Promoter and Three Novel Interacting Proteins Responding to Drought inPoncirus trifoliata
文献类型： 外文期刊
作者： Xiong, Jiang 1 ; Liu, Lian 1 ; Ma, Xiaochuan 1 ; Li, Feifei 1 ; Tang, Chaolan 1 ; Li, Zehang 1 ; Lu, Biwen 1 ; Zhou, Tie; 1 ;
作者机构： 1.Hunan Agr Univ, Dept Hort, Coll Hort, Changsha 410128, Peoples R China
2.Natl Ctr Citrus Improvement, Changsha 410128, Peoples R China
3.Hunan Acad Agr Sci, Inst Hort, Changsha 410125, Peoples R China
关键词： allene oxide synthase; promoter; Poncirus trifoliata; interaction
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN：
年卷期： 2020 年 21 卷 13 期
页码：
收录情况： SCI
摘要： Jasmonic acid (JA) plays a crucial role in various biological processes including development, signal transduction and stress response. Allene oxide synthase (AOS) catalyzing (13S)-hydroperoxyoctadecatrienoic acid (13-HPOT) to an unstable allene oxide is involved in the first step of JA biosynthesis. Here, we isolated the PtAOS1 gene and its promoter from trifoliate orange (Poncirus trifoliata). PtAOS1 contains a putative chloroplast targeting sequence in N-terminal and shows relative to pistachio (Pistacia vera) AOS. A number of stress-, light- and hormone-related cis-elements were found in thePtAOS1promoter which may be responsible for the up-regulation ofPtAOS1under drought and JA treatments. Transient expression in tobacco (Nicotiana benthamiana) demonstrated that the P-532(-532 to +1) fragment conferring drive activity was a core region in thePtAOS1promoter. Using yeast one-hybrid, three novel proteins, PtDUF886, PtDUF1685 and PtRAP2.4, binding to P(-532)were identified. The dual luciferase assay in tobacco illustrated that all three transcription factors could enhancePtAOS1promoter activity. GenesPtDUF1685andPtRAP2.4shared an expression pattern which was induced significantly by drought stress. These findings should be available evidence for trifoliate orange responding to drought through JA modulation.
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