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Quizalofop-p-ethyl resistance inPolypogon fugaxinvolves glutathione S-transferases

文献类型： 外文期刊

作者： Chen, Wen 1 ; Wu, Lamei 2 ; Wang, Junzhi 3 ; Yu, Qin 4 ; Bai, Lianyang 1 ; Pan, Lang 1 ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China

2.Hunan Acad Agr Sci, Hunan Agr Biotechnol Res Inst, Changsha 410125, Peoples R China

3.Hunan Univ, Longping Branch, Grad Sch, Changsha 410125, Peoples R China

4.Univ Western Australia, Sch Agr & Environm, Australian Herbicide Resistance Initiat, Crawley, WA 6009, Australia

关键词： ACCase; quizalofop-p-ethyl; haloxyfop-R-methyl; Polypogon fugax; nontarget-site resistance (NTSR); glutathione S-transferases (GST)

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2020 年 76 卷 11 期

页码：

收录情况： SCI

摘要： Background Asia minor bluegrass (Polypogon fugax) is one of the main weeds invading Chinese canola fields. TheP. fugaxresistant population SC-R, which survived quizalofop-p-ethyl at the field-recommended rate (67.5 g ha(-1)), was collected from a canola field in Qingsheng County in China. The present study aimed to (1) characterize the SC-R resistance pattern to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides, and (2) investigate the mechanism of quizalofop-p-ethyl resistance in this population. Results Dose-response studies showed that resistance to quizalofop-p-ethyl and haloxyfop occurred in the SC-R population. Four transcripts/genes encoding the plastidic ACCase carboxyl-transferase domain were isolated from theP. fugaxplants. No mutations in the four ACCase genes were detected in the SC-R population compared to the SC-S population. Pre-treatment with the known glutathione S-transferase (GST) inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl), reversed resistance to quizalofop-p-ethyl and partially reversed resistance to haloxyfop-R-methyl in the resistant population (SC-R). However, the cytochrome P450 inhibitor malathion did not reverse the resistance. There was no difference in basal GST activity (using CDNB as a substrate), but there was higher inducible GST activity in SC-R relative to SC-S. Two GST genes,GST2candGSTL3, were constitutively overexpressed in the resistant SC-R population. Conclusion This study confirmed that resistance to quizalofop-p-ethyl in the resistantP. fugaxpopulation is likely nontarget-site based involving GST, and this resistance mechanism also partially confers haloxyfop-R-methyl resistance.

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