文献类型： 外文期刊

作者： Peng Yajun ¹ ; Pan Lang ¹ ; Liu Ducai ² ; Cheng Xiaomei ³ ; Ma Guolan ² ; Li Sifu ² ; Liu Xueyuan ² ; Wang Lifeng ⁴ ; Bai Li ¹ ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha, Peoples R China

2.Hunan Acad Agr Sci, Inst Plant Protect, Changsha, Peoples R China

3.Hunan Acad Agr Sci, Inst Hort, Changsha, Peoples R China

4.Hunan Acad Agr Sci, Agr Biotechnol Res Inst, 2 Yuanda Rd, Changsha, Peoples R China

5.Hunan Acad Agr Sci, 2 Yuanda Rd, Changsha, Peoples R China

关键词： ACCase; amino acid substitution; dCAPS; resistance mechanism

期刊名称：WEED SCIENCE （ 影响因子：2.713； 五年影响因子：2.868 ）

ISSN： 0043-1745

年卷期： 2020 年 68 卷 3 期

页码：

收录情况： SCI

摘要： Chinese sprangletop [Leptochloa chinensis (L.) Nees] is one grass weed severely affecting rice (Oryza sativa L.) growth in paddies in China. Cyhalofop-butyl is the main herbicide used to control grass weeds in Chinese paddy fields, especially for controlling L. chinensis; however, L. chinensis has evolved resistance to cyhalofop-butyl due to continuous and extensive application. To investigate cyhalofop-butyl resistance levels and mechanisms in L. chinensis in some of the Chinese rice areas, 66 field populations were collected and treated with cyhalofop-butyl. Of these tested populations, 10 showed a high level of resistance to cyhalofop-butyl; the 50% effective dose ranged within 108.4 to 1,443.5 g ai ha(-1) with resistance index values of 9.1 to 121.8 when compared with the susceptible population. Acetyl-coenzyme A carboxylase genes (ACCase) of susceptible and all 10 resistant populations were amplified and sequenced. Among them, Ile-1781-Leu, Trp-2027-Cys, Trp-2027-Ser, and Ile-2041-Asn mutations were found in five resistant populations. No known resistance-related mutations were found in the other five resistant populations, indicating that resistance to cyhalofop-butyl in these populations was likely to be endowed by non-target site resistance mechanisms. Notably, the Ile-1781-Leu and Trp-2027-Cys substitutions have previously been reported, but this is the first report of Trp-2027-Ser and Ile-2041-Asn mutations in L. chinensis. Furthermore, three derived cleaved amplified polymorphic sequence methods were developed to rapidly detect these mutations in L. chinensis.