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Detoxification mechanism of herbicide in Polypogon fugax and its influence on rhizosphere enzyme activities

文献类型： 外文期刊

作者： Chen, Wen 1 ; Li, Sifu 2 ; Bai, Dingyi 1 ; Li, Zongfang 1 ; Liu, Haozhe 1 ; Bai, Lianyang 1 ; Pan, Lang 1 ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China

2.Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410125, Peoples R China

3.Huangpu Res Inst Longping Agr Sci & Technol, Guangzhou 510715, Peoples R China

关键词： Cytochrome P450 monooxygenase; Herbicide metabolism; Molecular docking; Soil enzyme activity; Rhizosphere

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 263 卷

页码：

收录情况： SCI

摘要： The excessive use of chemical herbicides has resulted in evolution of herbicide-resistant weeds. Cytochrome P450 monooxygenases (P450s) are vital detoxification enzymes for herbicide-resistant weeds. Herein, we confirmed a resistant (R) Polypogon fugax population showing resistance to quizalofop-p-ethyl, acetolactate synthase (ALS)-inhibiting herbicide pyroxsulam, and several other ACCase (acetyl-CoA carboxylase)-inhibiting herbicides. Molecular analysis revealed no target-site gene mutations in the R population. Foliar spraying with malathion clearly reversed the quizalofop-p-ethyl phytotoxicity. Higher level of quizalofop-p-ethyl degradation was confirmed in the R population using HPLC analysis. Subsequently, RNA-Seq transcriptome analysis indicated that the overexpression of CYP89A2 gene appeared to be responsible for reducing quizalofop-p-ethyl phytotoxicity. The molecular docking results supported a metabolic effect of CYP89A2 protein on most herbicides tested. Furthermore, we found that low doses of herbicides stimulated the rhizosphere enzyme activities in P. fugax and the increase of rhizosphere dehydrogenase of R population may be related to its resistance mechanism. In summary, our research has shown that metabolic herbicide resistance mediated by CYP89A2, contributes to quizalofop-p-ethyl resistance in P. fugax.

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