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Endocytic protein Pal1 regulates appressorium formation and is required for full virulence of Magnaporthe oryzae

文献类型： 外文期刊

作者： Chen, Deng 1 ; Hu, Hong 2 ; He, Wenhui 2 ; Zhang, Shimei 2 ; Tang, Mengxi 2 ; Xiang, Shikun 2 ; Liu, Caiyun 2 ; Cai, Xuan 2 ;

作者机构： 1.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China

2.Huazhong Agr Univ, State Key Lab Agr Microbiol, Coll Plant Sci & Technol, Wuhan, Peoples R China

3.Huazhong Agr Univ, Prov Key Lab Plant Pathol Hubei Prov, Coll Plant Sci & Technol, Wuhan, Peoples R China

关键词： actin ring; appressorium formation; autophagy; cAMP pathway; endocytosis; Pmk1 pathway

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN： 1464-6722

年卷期：

页码：

收录情况： SCI

摘要： Endocytosis plays key roles during infection of plant-pathogenic fungi, but its regulatory mechanisms are still largely unknown. Here, we identified a putative endocytosis-related gene, PAL1, which was highly expressed in appressorium of Magnaporthe oryzae, and was found to be important for appressorium formation and maturation. Deletion of PAL1 significantly reduced the virulence of M. oryzae due to defects in appressorial penetration and invasive growth in host cells. The Pal1 protein interacted and colocalized with the endocytosis protein Sla1, suggesting it is involved in endocytosis. The Delta pal1 mutant was significantly reduced in appressorium formation, which was recovered by adding exogenous cAMP and 3-isobutyl-1-methylxanthine (IBMX). Moreover, the phosphorylation level of Pmk1 in Delta pal1 was also reduced, suggesting Pal1 functions upstream of both the cAMP and Pmk1 signalling pathways. As a consequence, the utilization of glycogen and lipid, appressorial autophagy, actin ring formation, localization of septin proteins, as well as turgor accumulation were all affected in the Delta pal1 mutant. Taken together, Pal1 regulates cAMP and the Pmk1 signalling pathway for appressorium formation and maturation to facilitate infection of M. oryzae.

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