Cryptochrome 2 from Lilium x formolongi Regulates Photoperiodic Flowering in Transgenic Arabidopsis thaliana
文献类型： 外文期刊
作者： Wu, Xiao-Mei 1 ; Yang, Zheng-Min 1 ; Yang, Lin-Hao 1 ; Chen, Ji-Ren 1 ; Chen, Hai-Xia 1 ; Zheng, Si-Xiang 2 ; Zeng, Jian-Guo 1 ; Jia, Gui-Xia 4 ; Li, Yu-Fan 1 ;
作者机构： 1.Hunan Agr Univ, Coll Hort, Hunan Midsubtrop Qual Plant Breeding & Utilizat E, Changsha 410128, Peoples R China
2.Hunan Acad Agr Sci, Inst Agr Environm & Agro Ecol, Changsha 410125, Peoples R China
3.Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Coll Vet Med, Hunan Key Lab Tradit Chinese Vet Med, Changsha 410125, Peoples R China
4.Beijing Forestry Univ, Natl Engn Res Ctr Floriculture, Beijing Key Lab Ornamental Plants Germplasm Innov, Beijing Lab Urban & Rural Ecol Environm, Beijing 100083, Peoples R China
5.Beijing Forestry Univ, Coll Landscape Architecture, Beijing 100083, Peoples R China
关键词： cryptochrome; Lilium x formolongi; photoperiodic flowering
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2021 年 22 卷 23 期
页码：
收录情况： SCI
摘要： The photoperiodic flowering pathway is essential for plant reproduction. As blue and ultraviolet-A light receptors, cryptochromes play an important role in the photoperiodic regulation of flowering. Lilium x formolongi is an important cut flower that flowers within a year after seed propagation. Floral induction is highly sensitive to photoperiod. In this study, we isolated the CRYPTOCHROME2 gene (LfCRY2) from L. x formolongi. The predicted LfCRY2 protein was highly homologous to other CRY2 proteins. The transcription of LfCRY2 was induced by blue light. LfCRY2 exhibits its highest diurnal expression during the floral induction stage under both long-day and short-day photoperiods. Overexpression of LfCRY2 in Arabidopsis thaliana promoted flowering under long days but not short days, and inhibited hypocotyl elongation under blue light. Furthermore, LfCRY2 was located in the nucleus and could interact with L. x formolongi CONSTANS-like 9 (LfCOL9) and A. thaliana CRY-interacting basic-helix-loop-helix 1 (AtCIB1) in both yeast and onion cells, which supports the hypothesis that LfCRY2 hastens the floral transition via the CIB1-CO pathway in a manner similar to AtCRY2. These results provide evidence that LfCRY2 plays a vital role in promoting flowering under long days in L. x formolongi.
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