文献类型： 外文期刊

作者： Li, Bing ¹ ; Zhang, Xueli ² ; Liu, Zhiquan ³ ; Wang, Lulin ¹ ; Song, Liping ² ; Liang, Xiaomei ¹ ; Dou, Shengwei ¹ ; Tu, Jinxing ¹ ; Shen, Jinxiong ¹ ; Yi, Bin ¹ ; Wen, Jing ¹ ; Fu, Tingdong ¹ ; Dai, Cheng ¹ ; Gao, Changbin ² ; Wang, Aihua ² ; Ma, Chaozhi ¹ ;

作者机构： 1.Huazhong Agr Univ, Natl Sub Ctr Rapeseed Improvement Wuhan, Coll Plant Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

2.Wuhan Acad Agr Sci, Wuhan Vegetable Res Inst, Wuhan 430345, Peoples R China

3.Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha 410125, Peoples R China

关键词： Brassica rapa; self-incompatibility; self-compatibility; S haplotype; S locus receptor kinase (SRK); S locus cysteine-rich protein (SCR); amino acid variations

期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）

ISSN：

年卷期： 2021 年 10 卷 12 期

页码：

收录情况： SCI

摘要： Most flowering plants have evolved a self-incompatibility (SI) system to maintain genetic diversity by preventing self-pollination. The Brassica species possesses sporophytic self-incompatibility (SSI), which is controlled by the pollen- and stigma-determinant factors SP11/SCR and SRK. However, the mysterious molecular mechanism of SI remains largely unknown. Here, a new class II S haplotype, named BrS-325, was identified in a pak choi line '325', which was responsible for the completely self-compatible phenotype. To obtain the entire S locus sequences, a complete pak choi genome was gained through Nanopore sequencing and de novo assembly, which provided a good reference genome for breeding and molecular research in B. rapa. S locus comparative analysis showed that the closest relatives to BrS-325 was BrS-60, and high sequence polymorphism existed in the S locus. Meanwhile, two duplicated SRKs (BrSRK-325a and BrSRK-325b) were distributed in the BrS-325 locus with opposite transcription directions. BrSRK-325b and BrSCR-325 were expressed normally at the transcriptional level. The multiple sequence alignment of SCRs and SRKs in class II S haplotypes showed that a number of amino acid variations were present in the contact regions (CR II and CR III) of BrSCR-325 and the hypervariable regions (HV I and HV II) of BrSRK-325s, which may influence the binding and interaction between the ligand and the receptor. Thus, these results suggested that amino acid variations in contact sites may lead to the SI destruction of a new class II S haplotype BrS-325 in B. rapa. The complete SC phenotype of '325' showed the potential for practical breeding application value in B. rapa.