文献类型： 外文期刊
作者： Li, Hua 1 ; Deng, Lihua 1 ; Weng, Lvshui 1 ; Li, Jinjiang 1 ; Hu, Wenbin 3 ; Yu, Jianghui 1 ; Xiao, Youlun 3 ; Xiao, Guoying 1 ;
作者机构： 1.Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Hunan Acad Agr Sci, Changsha, Peoples R China
关键词： cell wall; cross-resistance; directional accumulation; fold higher; glyphosate; insecticidal protein; Lepidoptera pest; resistance
期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）
ISSN： 1526-498X
年卷期： 2024 年 80 卷 4 期
页码：
收录情况： SCI
摘要： BACKGROUND: The commercialized Bt (Bacillus thuringiensis) crops accumulate Bt protein within cells, but the intracellular interactions of foreign protein with endogenous protein inevitably result in large or small unintended effects. In this study, the Bt gene Cry1Ca was linked with the sequences of extracellular secretion signal peptide and carbohydrate binding module 11 to constitute a fusion gene SP-Cry1Ca-CBM11, and the fusion gene driven by constitutive promoters was used for secreting and anchoring onto the cell wall to minimize unintended effects. RESULTS: The transient expression in tobacco leaves demonstrated that the fusion protein was anchored on cell walls. The Cry1Ca contents of five homozygous rice transformants of single-copy insertion were different and descended in the order leaf > root > stem. The maximum content of Cry1Ca was 17.55 mu g g(-1) in leaves of transformant 21H037. The bioassay results revealed that the transformants exhibited high resistance to lepidopteran pests. The corrected mortality of pink stem borer (Sesamia inferens) and striped stem borer (Chilo suppressalis) ranged from 96.33% to 100%, and from 83.32% to 100%, respectively, and the corrected mortality of rice leaf roller (Cnaphalocrocis medinalis) was 92.53%. Besides, the agronomic traits of the five transformants were normal and similar to that of the recipient, and the transformants were highly resistant to glyphosate at the germination and seedling stages. CONCLUSION: The fusion Bt protein was accumulated on cell walls and endowed the rice with high resistance to lepidopteran pests without unintended effects in agronomic traits. (c) 2023 Society of Chemical Industry.
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