Plant-microbe hybrid synthesis provides new insights for the efficient use of Macleaya cordata
文献类型： 外文期刊
作者： Sun, Mengshan 1 ; Zhong, Xiaohong 2 ; Zhou, Li 3 ; Xu, Zixuan 1 ; Huang, Peng 5 ; Zeng, Jianguo 1 ;
作者机构： 1.Hunan Agr Univ, Hunan Key Lab Tradit Chinese Vet Med, Changsha, Hunan, Peoples R China
2.Hunan Agr Univ, Coll Hort, Changsha, Hunan, Peoples R China
3.Hunan Acad Agr Sci, Hunan Inst Agr Environm & Ecol, Changsha, Hunan, Peoples R China
4.Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China
5.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Hunan, Peoples R China
6.Hunan Agr Univ, Coll Vet Med, Changsha, Hunan, Peoples R China
7.Hunan Agr Univ, Natl & Local Union Engn Res Ctr Vet Herbal Med Re, Changsha, Hunan, Peoples R China
关键词： Bioconversion; Chelerytrine; Macleaya cordata; Sanguinarine
期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：4.253； 五年影响因子：4.272 ）
ISSN： 0959-3993
年卷期： 2022 年 38 卷 6 期
页码：
收录情况： SCI
摘要： Sanguinarine and chelerytrine have antibacterial and anti-inflammatory effects and is the main active ingredients of growth promoters in animals. Currently, Sanguinarine and chelerytrine were extracted from the capsules of the medicinal plant Macleaya cordata. However, the biomass of M. cordata nonmedicinal parts (leaves) accounted for a large proportion and contained a rich presentation of protopine and allocryptopine which are the precursor compounds of sanguinarine and chelerytrine. The aim of this study was to develop a new method for producing sanguinarine and chelerytrine through yeast transformation of protopine and allocryptopine in M. cordata leaves. First, we isolated different genes from Papaver somniferum (PsP6H, PsCPR, PsDBOX), Eschscholtzia californica (EcP6H), Cucumis sativus (CuCPR), Arabidopsis thaliana (AtCPR) and M. cordata (Mc11229, Mc11218, Mc6408, Mc6407, Mc19967, Mc13802). Additionally, some of the gene sequences were codon optimized. Then, we transformed these genes into yeast cells to compare the catalytic efficiency. Second, we used the most efficient strains to biotransform the leaves of M. cordata. Finally, we obtained 85.415 +/- 11.887 ng mL(-1) sanguinarine and 4.288 +/- 1.395 ng mL(-1) chelerytrine, which was more than 2-3 times the content in leaves of M. cordata. Overall, we using the nonmedicinal parts of M. cordata and successfully obtained sanguinarine and chelerytrine by the plant-microbial hybrid synthesis method.
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