Elucidation of miRNAs-Mediated Responses to Low Nitrogen Stress by Deep Sequencing of Two Soybean Genotypes
文献类型： 外文期刊
作者： Wang, Yejian 2 ; Zhang, Chanjuan 2 ; Hao, Qinnan 2 ; Sha, Aihua 2 ; Zhou, Rong 2 ; Zhou, Xinan 2 ; Yuan, Longping 1 ;
作者机构： 1.Cent S Univ, Long Ping Branch, Grad Sch, Changsha, Hunan, Peoples R China
2.Chinese Acad Agr Sci, Key Lab Oil Crop Biol, Minist Agr, Oil Crops Res Inst, Wuhan, Peoples R China
3.Natl Hybrid Rice R&D Ctr, Changsha, Hunan, Peoples R China
4.Chinese Acad Agr Sci, Grad Sch, Beijing 100193, Peoples R China
5.Hunan Acad Agr Sci, Inst Crops Res, Changsha, Hunan, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2013 年 8 卷 7 期
页码：
收录情况： SCI
摘要： Nitrogen (N) is a major limiting factor in crop production, and plant adaptive responses to low N are involved in many post-transcriptional regulation. Recent studies indicate that miRNAs play important roles in adaptive responses. However, miRNAs in soybean adaptive responses to N limitation have been not reported. We constructed sixteen libraries to identify low N-responsive miRNAs on a genome-wide scale using samples from 2 different genotypes (low N sensitive and low N tolerant) subjected to various periods of low nitrogen stress. Using high-throughput sequencing technology (Illumina-Solexa), we identified 362 known miRNAs variants belonging to 158 families and 90 new miRNAs belonging to 55 families. Among these known miRNAs variants, almost 50% were not different from annotated miRNAs in miRBase. Analyses of their expression patterns showed 150 known miRNAs variants as well as 2 novel miRNAs with differential expressions. These differentially expressed miRNAs between the two soybean genotypes were compared and classified into three groups based on their expression patterns. Predicted targets of these miRNAs were involved in various metabolic and regulatory pathways such as protein degradation, carbohydrate metabolism, hormone signaling pathway, and cellular transport. These findings suggest that miRNAs play important roles in soybean response to low N and contribute to the understanding of the genetic basis of differences in adaptive responses to N limitation between the two soybean genotypes. Our study provides basis for expounding the complex gene regulatory network of these miRNAs.
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