Heterologous expression of Helicoverpa armigera cytochrome P450 CYP6B7 in Pichia pastoris and interactions of CYP6B7 with insecticides
文献类型： 外文期刊
作者： Zhao, Chunqing 2 ; Song, Genmiao 1 ; Duan, Hongxia 1 ; Tang, Tao 1 ; Wang, Chen 1 ; Qiu, Lihong 1 ;
作者机构： 1.China Agr Univ, Coll Sci, Beijing, Peoples R China
2.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China
3.Hunan Acad Agr Sci, Inst Plant Protect, Changsha, Hunan, Peoples R China
关键词： CYP6B7;Helicoverpa armigera;heterologous expression;interaction;molecular docking;Pichia pastoris
期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）
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收录情况： SCI
摘要： BACKGROUNDPrevious studies indicated that constitutive over-expression of cytochrome P450 CYP6B7 was involved in fenvalerate resistance in Helicoverpa armigera. In this study, the CYP6B7 gene from H. armigera (namely HaCYP6B7), was heterologously expressed in Pichia pastoris GS115. A vector pPICZA-HaCYP6B7 was constructed and transformed into P. pastoris GS115, the transformant of pPICZA-HaCYP6B7-GS115 was then cultured and induced by 1% (v/v) methanol and the heterologous expression of HaCYP6B7 protein in P. pastoris was confirmed by SDS-PAGE and western blot.
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