文献类型： 外文期刊

作者： Liang, Zeng-Enni ¹ ; Yi, You-Jin ² ; Guo, Yu-Tong ³ ; Wang, Ren-Cai ¹ ; Hu, Qiu-Long ¹ ; Xiong, Xing-Yao ¹ ;

作者机构： 1.Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Hunan, Peoples R China

2.Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China

3.State Adm Tradit Chinese Med, State Key Lab Subhlth Intervent Technol, Changsha 410128, Hunan, Peoples R China

关键词： Ganoderma lucidum polysaccharides;human colon cancer;apoptosis;death receptor pathway

期刊名称：MOLECULAR MEDICINE REPORTS （ 影响因子：2.952； 五年影响因子：2.754 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAE-cellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLP-mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases-3, -8 and -9 was involved in GLP-stimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase-3 proteins, whilst reducing the expression of cleaved poly(ADP-ribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs.