您好，欢迎访问湖南省农业科学院 机构知识库！

Cloning and characterisation of NADPH-dependent cytochrome P450 reductase gene in the cotton bollworm, Helicoverpa armigera

文献类型： 外文期刊

作者： Zhao, Chunqing 1 ; Tang, Tao 1 ; Feng, Xiaoyun 1 ; Qiu, Lihong 1 ;

作者机构： 1.China Agr Univ, Coll Sci, Beijing 100193, Peoples R China

2.Hunan Acad Agr Sci, Inst Plant Protect, Changsha, Hunan, Peoples R China

关键词： Helicoverpa armigera;cytochrome P450 enzyme systems (P450 systems);NADPH-dependent cytochrome P450 reductase (CPR);real-time quantitative polymerase chain reaction (RT-qPCR);phenobarbital (PB)

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： BACKGROUND: Previous studies in our laboratory showed that cytochrome P450 CYP6B7 plays a critical role in a Handan fenvalerate resistant strain (HDFR) of Helicoverpa armigera. As an important component of P450 enzyme systems, cytochrome P450 reductase (CPR) plays an essential role in transferring electrons from NADPH to the P450-substrate complex. However, little information about CPR in H. armigera (HaCPR) has been reported. RESULTS: A full-length cDNA (3525 bp) of HaCPR was cloned. The open reading frame of the HaCPR gene encoded 687 amino acids and shared 27.87-95.21% identities with other known CPRs. Bioinformatic analysis showed that HaCPR is a transmembrane protein with Mw of 77.4 kDa and contains conserved features. The results of real-time quantitative polymerase chain reaction showed that the expression level of HaCPR mRNA was 1.84-fold higher in midgut of 5th instars of the Handan susceptible strain than that in pupae, and the level in the midgut of HDFR strain was 2.02-fold higher than that of the Handan susceptible strain. The levels of HaCPR mRNA were induced by phenobarbital at concentrations of 2 and 4 mg g~(-1), which enhanced 5.20- and 17.45-fold, respectively, compared to that of the control after 48 h of phenobarbital treatment. CONCLUSIONS: The results indicate that HaCPR is important for the development of H. armigera and may play an essential role in the P450-mediated insecticide resistance of H. armigera to fenvalerate.

  • 相关文献

[1]Identification and expression analysis of NADH-cytochrome b(5) reductase gene in the cotton bollworm, Helicoverpa armigera. Tang, Tao,Feng, Xiaoyun,Qiu, Lihong,Tang, Tao,Liu, Jiqin. 2012

[2]RNA Interference-mediated Knockdown of Several Components of Cytochrome P450 Enzyme Systems, from the Cotton Bollworm,Helicoverpa armigera , Enhances Larval Susceptibility to beta-Cypermethrin. Tao Tang,Chunqing Zhao,Xiaoyun Feng,Xueyuan Liu,Lihong Qiu. 2012

[3]Heterologous expression of Helicoverpa armigera cytochrome P450 CYP6B7 in Pichia pastoris and interactions of CYP6B7 with insecticides. Song, Genmiao,Duan, Hongxia,Tang, Tao,Wang, Chen,Qiu, Lihong,Zhao, Chunqing,Tang, Tao.

[4]Cloning and expression of cytochrome P450 CYP6B7 in fenvalerate-resistant and susceptible Helicoverpa armigera (Hubner) from China. Tang, T.,Cheng, Y.,Shui, R.,Zhang, W.,Qiu, L.,Zhang, H.,Tang, T.. 2010

[5]Knockdown of several components of cytochrome P450 enzyme systems by RNA interference enhances the susceptibility of Helicoverpa armigera to fenvalerate. Zhao, Chunqing,Feng, Xiaoyun,Qiu, Lihong,Tang, Tao,Liu, Xueyuan. 2012

[6]Effects of branched-chain amino acid supplementation on fecundity, lifespan and flight ability of Helicoverpa armigera. Wang, Lifeng,Yuan, Zheming,Zhou, Shihao,Wang, Lifeng.

[7]Factors affecting larval cannibalism in the cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae). Zhao, Chunqing,Xu, Li,Qiu, Lihong,Zhao, Chunqing.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台