文献类型： 外文期刊

作者： Huang, Yue ¹ ; He, Jiaxian ¹ ; Xu, Yuantao ¹ ; Zheng, Weikang ¹ ; Wang, Shaohua ³ ; Chen, Peng ⁴ ; Zeng, Bin ⁴ ; Yang, Shuizhi ⁴ ; Jiang, Xiaolin ¹ ; Liu, Zishuang ¹ ; Wang, Lun ¹ ; Wang, Xia ¹ ; Liu, Shengjun ¹ ; Lu, Zhihao ¹ ; Liu, Ziang ¹ ; Yu, Huiwen ¹ ; Yue, Jianqiang ³ ; Gao, Junyan ³ ; Zhou, Xianyan ³ ; Long, Chunrui ³ ; Zeng, Xiuli ⁵ ; Guo, Yong-Jie ⁶ ; Zhang, Wen-Fu ⁸ ; Xie, Zongzhou ¹ ; Li, Chunlong ¹ ; Ma, Zhaocheng ¹ ; Jiao, Wenbiao ¹ ; Zhang, Fei ¹ ; Larkin, Robert M. ¹ ; Krueger, Robert R. ⁹ ; Smith, Malcolm W. ¹⁰ ; Ming, Ray ¹¹ ; Deng, Xiuxin ¹ ; Xu, Qiang ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry, Natl Key Lab Germplasm Innovat & Utilizat Crops, Wuhan 430070, Peoples R China

2.Hubei Hongshan Lab, Wuhan, Peoples R China

3.Yunnan Acad Agr Sci, Inst Trop & Subtrop Cash Crops, Baoshan, Yunnan, Peoples R China

4.Hunan Acad Agr Sci, Hort Inst, Changsha, Peoples R China

5.Minist Agr & Rural Affairs, Qinghai Tibet Plateau Fruit Trees Sci Observat Tes, Lhasa, Qinghai, Peoples R China

6.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Peoples R China

7.Univ Chinese Acad Sci, Beijing, Peoples R China

8.Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Mengla, Peoples R China

9.US Dept Agr Agr Res Serv, Natl Clonal Germplasm Repository Citrus & Dates, Riverside, CA 92507 USA

10.Dept Agr & Fisheries, Bundaberg, Qld, Australia

11.Univ Illinois, Dept Plant Biol, Urbana, IL USA

期刊名称：NATURE GENETICS （ 影响因子：30.8； 五年影响因子：37.4 ）

ISSN： 1061-4036

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The orange subfamily (Aurantioideae) contains several Citrus species cultivated worldwide, such as sweet orange and lemon. The origin of Citrus species has long been debated and less is known about the Aurantioideae. Here, we compiled the genome sequences of 314 accessions, de novo assembled the genomes of 12 species and constructed a graph-based pangenome for Aurantioideae. Our analysis indicates that the ancient Indian Plate is the ancestral area for Citrus-related genera and that South Central China is the primary center of origin of the Citrus genus. We found substantial variations in the sequence and expression of the PH4 gene in Citrus relative to Citrus-related genera. Gene editing and biochemical experiments demonstrate a central role for PH4 in the accumulation of citric acid in citrus fruits. This study provides insights into the origin and evolution of the orange subfamily and a regulatory mechanism underpinning the evolution of fruit taste. De novo genome assemblies of 12 species from Aurantioideae and pangenome analyses provide insights into the origin and evolution of the orange subfamily and the genetic basis of flavor in citrus fruits.