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Construction of high-yielding edeine strains and an initial exploration of their control efficacy against crop pathogens

文献类型： 外文期刊

作者： Zhang, Liang 1 ; Chen, Ziyue 1 ; Xia, Fei 3 ; Liu, Tianbo 4 ; Liu, Qingshu 2 ; Chen, Wu 1 ;

作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China

2.Hunan Acad Agr Sci, Inst Microbiol, Yuelushan Lab, Changsha 410125, Peoples R China

3.Cent South Univ Forestry & Technol, Changsha 410000, Peoples R China

4.Hunan Tobacco Sci Res Inst, Changsha 410004, Peoples R China

关键词： Brevibacillus brevis; Edeine; Transcriptional regulator; Promoter; Genetic modification; Biocontrol; Ralstonia solanacearum

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：5.2； 五年影响因子：6.1 ）

ISSN：

年卷期： 2025 年 12 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundEdeine, a non-ribosomal antibiotic produced by Brevibacillus brevis X23, has a broad-spectrum antimicrobial activity against plant pathogens, but its low yield in wild-type strains limits its potential for agricultural applications. This study aimed to enhance edeine production by genetically engineering B. brevis X23.MethodsRed/ET homologous recombination technology was used to construct engineered strain X23(Delta abrB)::Pmwp by knocking out global negative regulator AbrB and replacing the natural promoter of the edeine biosynthesis gene cluster (ede BGC) with the strong Pmwp promoter.ResultsQuantitative PCR revealed significantly increased ede BGC transcription levels in X23(Delta abrB)::Pmwp compared to the wild-type strain. High-performance liquid chromatography-mass spectrometry (HPLC-MS) demonstrated a 10.1-fold increase in the edeine peak area with the final yield reaching 97.3 mg/L. In pot experiments for tobacco bacterial wilt (pathogen name Ralstonia solanacearum) control, X23(Delta abrB)::Pmwp showed an efficacy of 82.9%, representing a 32.6% improvement over the wild-type strain (62.5%). The engineered strain also demonstrated an increased plate inhibition capacity of 20.5-60.9% against Verticillium dahliae Kleb, Rhizoctonia solani, and Fusarium oxysporum in cotton, indicating its potential application in crop protection.ConclusionsTherefore, this study yielded an engineered strain with increased edeine production and enhanced biocontrol efficacy, contributing to the development of biological control methods for plant diseases.

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