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Systematic characterization of the ubiquitin-specific proteases in Magnaporthe oryzae

文献类型： 外文期刊

作者： Cai, Xuan 1 ; Wang, Zhao 1 ; Hou, Yuxi 1 ; Liu, Caiyun 1 ; Hendy, Ahmed 1 ; Xing, Junjie 3 ; Chen, Xiao-Lin 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Prov Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Peoples R China

2.Alexandria Univ, Fac Agr Saba Basha, Dept Agr Bot, Alexandria 21513, Egypt

3.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410082, Peoples R China

关键词： Ubiquitin; Deubiquitinases; Ubiquitin-specific proteases; Infection process; Gene family; Magnaporthe oryzae

期刊名称：PHYTOPATHOLOGY RESEARCH （ 影响因子：3.955； 五年影响因子：3.955 ）

ISSN： 2096-5362

年卷期： 2020 年 2 卷 1 期

页码：

收录情况： SCI

摘要： Ubiquitin-specific protease (UBP) family is the largest group of deubiquitinases, which plays important roles in eukaryotic organisms. Comprehensive analysis of UBP genes has not been conducted in the plant pathogenic fungi. In this study, 11 putative UBP genes were identified and characterized in the rice blast fungus Magnaporthe oryzae. Expression profile analysis showed that UBP3, UBP6, UBP12 and UBP14 were highly expressed in different tissues of M. oryzae. In all ubp mutants, especially Delta ubp3, Delta ubp12 and previously reported Delta ubp14, the ubiquitination levels were evidently elevated, which is consistent with their molecular roles in de-ubiquitination. The Delta ubp1, Delta ubp3, Delta ubp4, Delta ubp8 and Delta ubp14 mutants were reduced in colony growth. Most of the ubp mutants were severely reduced in conidia production capacity, indicating important roles of the UBPs in conidia formation. Except for Delta ubp2 and Delta ubp16, all of the other mutants were decreased in virulence to host plants and defective in invasive growth. These ubp mutants also induced massive ROS accumulation in host cells. We also found that the UBPs may function as both positive and negative regulators in stress response and nutrient utilization of M. oryzae. Collectively, UBPs are important for development, stress response, nutrient utilization and infection of M. oryzae.

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