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Effects of Rhizosphere and Long-Term Fertilization Practices on the Activity and Community Structure of Denitrifiers Under Double-Cropping Rice Field

文献类型： 外文期刊

作者： Tang, Haiming 1 ; Xiao, Xiaoping 1 ; Li, Chao 1 ; Tang, Wenguang 1 ; Cheng, Kaikai 1 ; Wang, Ke 1 ; Pan, Xiaochen 1 ; Li, 1 ;

作者机构： 1.Hunan Acad Agr Sci, Inst Soil & Fertilizer, Changsha 410125, Hunan, Peoples R China

关键词： Rice; long-term fertilization; rhizosphere; denitrification; soil denitrification rates; community composition

期刊名称：COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS （ 影响因子：1.327； 五年影响因子：1.315 ）

ISSN： 0010-3624

年卷期： 2019 年 50 卷 6 期

页码：

收录情况： SCI

摘要： The soil physicochemical properties, soil denitrification rates (PDR), denitrifiers via nitrite reductases (nirK and nirS) and nitrous oxide reductase (nosZ), abundance and community composition of denitrifiers in both the rhizosphere and bulk soil from a long-term (32 year) fertilizer field experiment conducted during late rice season were investigated by using the MiSeq sequencing, quantitative PCR, terminal restriction fragment polymorphism (T-RFLP). The experiment including four treatments: without fertilizer input (CK), chemical fertilizer alone (MF), rice straw residue and chemical fertilizer (RF), and organic manure and chemical fertilizer (OM). The results showed that the application of rice straw residue and organic manure increased soil organic carbon (C), total nitrogen (N), and NH4+-N contents. The nirK, nirS, and nosZ copy numbers with OM and RF treatments were significant higher than that of the MF and CK treatments in the rhizosphere and bulk soil (p <0.05). The principal coordinate analysis (PCoA) analysis showed that the different parts of root zone are the most important factors for the variation of denitrifying bacteria community, and the different fertilization treatments is the second important factors for the variation of denitrifying bacteria community. The MiSeq sequencing result showed that nirK, nirS and nosZ-type denitrifiers communities within bulk soil had lower species diversity compared with rhizosphere soil, and were dominated by Rhizobiales, Rhodobacterales, Burkholderiales, and Pseudomonadales. As a result, the application of fertilization practices had significant effects on soil N and PDR levels, and affected the abundance and community composition of N-functional microbes.

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