Identification of the ionotropic GABA receptor-like subunits from the striped stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae)
文献类型： 外文期刊
作者： Jia, Zhong-Qiang 1 ; Sheng, Cheng-Wang 1 ; Tang, Tao 2 ; Liu, Di 1 ; Leviticus, Kipchoge 1 ; Zhao, Chun-Qing 1 ; Chang 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Educ Minist, Nanjing 2100, Jiangsu, Peoples R China
2.Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410125, Hunan, Peoples R China
3.Shanghai Acad Agr Sci, Shanghai Engn Res Ctr Low Carbon Agr, Ecoenvironm Protect Res Inst, Shanghai 201403, Peoples R China
关键词： 8916; Chilo suppressalis; Fluralaner; GABA receptor; RDL; LCCH3
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）
ISSN： 0048-3575
年卷期： 2019 年 155 卷
页码：
收录情况： SCI
摘要： Ionotropic gamma-aminobutyric acid (GABA) receptors (GABARs) mediate rapid inhibitory neurotransmission in both vertebrates and invertebrates, and are important molecular targets of insecticides. However, components of insect GABARs remain elusive. In addition to CsRDL1 and CsRDL2, the complementary DNAs (cDNAs) of another two GABA receptor-like subunits, CsLCCH3 and Cs8916, were identified from the rice striped stem borer, Chilo suppressalis Walker in the present study. Both CsLCCH3 and Cs8916 subunits shared common structural features, such as a highly-conserved Cys-loop structure, six distinct regions involved in ligand binding (loops A-F), and four transmembrane domains (TM 1-4). Transcript analysis demonstrated that the relative mRNA expression levels of both CsLCCH3 and Cs8916 subunits were the highest in the ventral nerve cord. Regarding developmental stage, transcript levels of both subunits were highest in eggs. Injections of double-stranded RNAs (dsRNAs), including dsRDL1, dsRDL2, dsLCCH3, or ds8916, significantly reduced mRNA abundance after 24 and 48 h. However, no observable effects on the development of C. suppressalis were observed. Injection of dsRDL1 or dsRDL2 did significantly reduce the mortality of C. suppressalis treated with fluralaner. Our results indicated that CsRDLs mediated the susceptibility of C. suppressalis to fluralaner, whereas CsLCCH3 and CsL8916 did not. The current investigation enhances our knowledge of Lepidopteran GABARs and offers a molecular basis for the development of novel insecticides to control C. suppressalis.
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