文献类型： 外文期刊

作者： Hu Wen-bin ³ ; Deng Xiang-yang ¹ ; Deng Xiao-xiang ² ; Deng Li-hua ¹ ; Xiao You-lun ⁴ ; He Xing-jian ⁵ ; Fu Xi-qin ² ; Xi ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China

2.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Hunan Acad Agr Sci, Hunan Plant Protect Inst, Changsha 410125, Hunan, Peoples R China

5.Sericultural Res Inst Hunan Prov, Changsha 410125, Hunan, Peoples R China

关键词： rice; resistance; glufosinate; rice leaf roller; brown planthopper; bacterial blight

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 3 期

页码：

收录情况： SCI

摘要： In this study, the characteristic of three transformants named as B1C106-1, B1C106-2, and B1C106-3 were studied that carried three innate resistant genes Bph14, Bph15, and Xa23, and two enthetic resistant genes Cry1Ca(#) and Bar. The five resistant genes were all verified by PCR and the two enthetic genes were identified in single copy insertion by Southern blot. At tillering stage, the Cry1C and PAT (phosphinothricin acetyl transferase) protein contents in leaf, sheath, and stem of T-2 generation were in the similar pattern: leaf>stem>sheath, and showed significant difference (P<0.01) among three organs. The average contents of Cry1C protein in plant of B1C106-1, B1C106-2, and B1C106-3 were 12.95, 6.57, and 11.30 mu g g(-1), respectively, and showed significant difference (P<0.01) among them. However, the average contents of PAT in plant of B1C106-1, B1C106-2, and B1C106-3 were 28.54, 27.66, and 28.02 mu g g(-1), respectively, and there were no significant difference among three transformants. The glufosinate tolerable concentration of three transformants of T-3 generation reached at least 6 g L-1, and the mortality of rice leaf rollers were above 97.4% in 5 days after being fed with fresh transformants' leaves. The Cry1C protein toxicity was also assessed by silkworms, and the mortality of silkworms feeding mulberry leaves smeared with Cry1C protein extracts of leaves of B1C106-1, B1C106-2, and B1C106-3 were 90, 67.8, and 87.8%, respectively, that were positive correlation (r=0.993) with Cry1C protein contents in plant of three transformants. The three transformants also maintained high resistance to brown planthopper and bacterial blight as the original version. The above results indicate the tetra-resistant rice germplasm was well-developed by pyramiding innate and enthetic resistant genes in an elite line to provide with resistances of glufosinate, rice leaf roller, brown planthopper, and bacterial blight.