Function analysis of CYP321A9 from Spodoptera frugiperda (Lepidoptera: Noctuidae) associated with emamectin benzoate, and a novel insecticide, cyproflanilide detoxification
文献类型： 外文期刊
作者： Shi, Yang 1 ; He, Li 1 ; Ding, Wenbing 2 ; Huang, Hong 4 ; He, Hualiang 1 ; Xue, Jin 1 ; Gao, Qiao 1 ; Zhang, Zhixiang 5 ; Li, Youzhi 1 ; Qiu, Lin 1 ;
作者机构： 1.Hunan Agr Univ, Coll Plant Protect, Hunan Prov Key Lab Biol & Control Plant Dis & Inse, Changsha 410128, Peoples R China
2.Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct Ing, Changsha 410128, Peoples R China
3.Hunan Agr Univ, Hunan Prov Engn & Technol Res Ctr Biopesticide & F, Changsha 410128, Peoples R China
4.Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410125, Peoples R China
5.South China Agr Univ, Coll Plant Protect, Guangzhou 510642, Peoples R China
关键词： cyproflanilide; emamectin benzoate; detoxification
期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）
ISSN： 0022-0493
年卷期： 2023 年
页码：
收录情况： SCI
摘要： The fall armyworm, Spodoptera frugiperda, is an invasive agricultural pest that is a serious threat to agricultural production and global food security. Chemical control is the most effective method for preventing outbreaks of S. frugiperda. However, insecticide resistance often develops as a result of prolonged pesticide use, and the molecular mechanisms involved in insecticide resistance remain unclear. Insect cytochrome P450 monooxygenases play an important role in the detoxification of insecticides and insecticide resistance in Lepidoptera. In our study, the LC50 of a novel insecticide (cyproflanilide) and a conventional insecticide (emamectin benzoate) for S. frugiperda second-instar larvae were 7.04 and 1.61 mg/L, respectively. Furthermore, CYP321A9 expression was upregulated in larvae exposed to these insecticides. Additionally, knockdown of CYP321A9 by feeding larvae with dsRNA for 72 h significantly increased the mortality of S. frugiperda exposed to emamectin benzoate and cyproflanilide by 23.33% and 7.78%, respectively. Our results indicate that CYP321A9 may play an important role in the detoxification of emamectin benzoate and cyproflanilide in S. frugiperda. Our findings provide a basis to better understand the mechanisms of insecticide resistance and contribute to the control of S. frugiperda. Graphical Abstract
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