Comparative Analysis of Heat-Tolerant and Heat-Susceptible Rice Highlights the Role of OsNCED1 Gene in Heat Stress Tolerance
文献类型： 外文期刊
作者： Zhou, Huang 1 ; Wang, Yingfeng 1 ; Zhang, Yijin 1 ; Xiao, Yunhua 1 ; Liu, Xiong 1 ; Deng, Huabing 1 ; Lu, Xuedan 1 ; Tang, Wenbang 1 ; Zhang, Guilian 1 ;
作者机构： 1.Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China
2.Hunan Agr Univ, Hunan Prov Key Lab Rice & Rapeseed Breeding Dis R, Changsha 410128, Peoples R China
3.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China
关键词： rice; heading and flowering stage; heat stress; transcriptome; OsNCED1; antioxidant enzyme
期刊名称：PLANTS-BASEL （ 影响因子：4.658； 五年影响因子：4.827 ）
ISSN：
年卷期： 2022 年 11 卷 8 期
页码：
收录情况： SCI
摘要： To elucidate the mechanism underlying the response of rice to heat stress (HS), the transcriptome profile of panicles was comparatively analyzed between the heat-tolerant line 252 (HTL252) and heat-susceptible line 082 (HSL082), two rice recombinant inbred lines (RILs). Our differentially expressed gene (DEG) analysis revealed that the DEGs are mainly associated with protein binding, catalysis, stress response, and cellular process. The MapMan analysis demonstrated that the heat-responsive (HR) genes for heat shock proteins, transcription factors, development, and phytohormones are specifically induced in HTL252 under HS. Based on the DEG analysis, the key gene OsNCED1 (0s02g0704000), which was induced under HS, was selected for further functional validation. Moreover, 9-cis-epoxycarotenoid dioxygenase (NCED) is a key rate-limiting enzyme in the ABA biosynthetic pathway. Overexpression of OsNCED1 improved the HS tolerance of rice at the heading and flowering stage. OsNCED1-overexpression plants exhibited significant increases in pollen viability, seed setting rate, superoxide dismutase (SOD) and peroxidase (POD) activities, while significantly lower electrolyte leakage and malondialdehyde (MDA) content relative to the wild type (WT). These results suggested that OsNCED1 overexpression can improve the heat tolerance of rice by enhancing the antioxidant capacity. Overall, this study lays a foundation for revealing the molecular regulatory mechanism underlying the response of rice to prolonged HS.
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