文献类型： 外文期刊

作者： Zhang, Xia ¹ ; Zhang, Li ² ; Zhang, Damao ² ; Su, Dingding ⁶ ; Li, Weidong ⁵ ; Wang, Xiangfei ² ; Chen, Qianru ² ; Cai, Wenqi ² ; Xu, Lu ² ; Cao, Fuxiang ² ; Zhang, Dongling ⁸ ; Yu, Xiaoying ² ; Li, Yanlin ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

2.Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China

3.Minist Educ, Engn Res Ctr Hort Crop Germplasm Creat & New Varie, Changsha 410128, Peoples R China

4.Hunan Midsubtrop Qual Plant Breeding & Utilizat En, Changsha 410128, Peoples R China

5.Hunan Acad Agr Sci, Hunan Hort Res Inst, Changsha 410125, Peoples R China

6.Peking Univ, Inst Adv Agr Sci, Weifang 262041, Peoples R China

7.Hunan Key Lab Innovat & Comprehens Utilizat, Changsha 410128, Peoples R China

8.Univ Georgia, Dept Hort, Athens, GA 30602 USA

关键词： Leaf colour; Pigmentation; Anthocyanin; Synthesis pathway; Loropetalum chinense var; Rubrum

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.3； 五年影响因子：5.9 ）

ISSN： 1471-2229

年卷期： 2023 年 23 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundLoropetalum chinense var. rubrum (L. chinense var. rubrum) is a precious, coloured-leaf native ornamental plant in the Hunan Province. We found an L. chinense var. rubrum tree with three different leaf colours: GL (green leaf), ML (mosaic leaf), and PL (purple leaf). The mechanism of leaf coloration in this plant is still unclear. Therefore, this study aimed to identify the metabolites and genes involved in determining the colour composition of L. chinense var. rubrum leaves, using phenotypic/anatomic observations, pigment content detection, and comparative metabolomics and transcriptomics.ResultsWe observed that the mesophyll cells in PL were purple, while those in GL were green and those in ML were a mix of purple-green. The contents of chlorophyll a, b, carotenoids, and total chlorophyll in PL and ML were significantly lower than those in GL. While the anthocyanin content in PL and ML was significantly higher than that in GL. The metabolomics results showed the differences in the content of cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, cyanidin 3,5-O-diglucoside, pelargonidin, and petunidin 3,5-diglucoside in ML, GL, and PL were significant. Considering that the change trend of anthocyanin content change was consistent with the leaf colour difference, we speculated that these compounds might influence the colour of L. chinense var. rubrum leaves. Using transcriptomics, we finally identified nine differentially expressed structural genes (one ANR (ANR1217); four CYP75As (CYP75A1815, CYP75A2846, CYP75A2909, and CYP75A1716); four UFGTs (UFGT1876, UFGT1649, UFGT1839, and UFGT3273) and nine transcription factors (two MYBs (MYB1057 and MYB1211), one MADS-box (MADS1235), two AP2-likes (AP2-like1779 and AP2-like2234), one bZIP (bZIP3720), two WD40s (WD2173 and WD1867) and one bHLH (bHLH1631) that might be related to flavonoid biosynthesis and then impacted the appearance of colour in L. chinense var. rubrum leaves.ConclusionThis study revealed potential molecular mechanisms associated with leaf coloration in L. chinense var. rubrum by analyzing differential metabolites and genes related to the anthocyanin biosynthesis pathway. It also provided a reference for research on leaf colour variation in other ornamental plants.